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TIFA Polyclonal antibody

TIFA Polyclonal Antibody for WB, ELISA

Cat No. 11333-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

T2BP, T6BP, TIFA, TIFAA, TRAF2 binding protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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WB analysis using 11333-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11333-1-AP (TIFA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in

K-562 cells, L02 cells, TT cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11333-1-AP targets TIFA in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1876 Product name: Recombinant human TIFA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-184 aa of BC014259 Sequence: MTSFEDADTEETVTCLQMTVYHPGQLQCGIFQSISFNREKLPSSEVVKFGRNSNICHYTFQDKQVSRVQFSLQLFKKFNSSVLSFEIKNMSKKTNLIVDSRELGYLNKMDLPYRCMVRFGEYQFLMEKEDGESLEFFETQFILSPRSLLQENNWPPHRPIPEYGTYSLCSSQSSSPTEMDENES Predict reactive species
Full Name	TRAF-interacting protein with forkhead-associated domain
Calculated Molecular Weight	21 kDa
Observed Molecular Weight	21 kDa
GenBank Accession Number	BC014259
Gene Symbol	TIFA
Gene ID (NCBI)	92610
RRID	AB_3669126
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96CG3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIFA (TRAF-interacting protein with FHA domain-containing protein A ), also called T2BP, can interact with TRAF6 and plays an important role in innate immunity (PMID: 32910997; 35635239). Oligomerization of TIFA, which induces oligomerization and polyubiquitinylation of TRAF6, in turn activates TAK and IKK, finally mediates NF‐κB activation in the Toll‐like receptor 4/interleukin‐1 signaling pathway (PMID: 15492226; 22566686). It has been reported that TIFA also plays various roles in different cancer types (PMID: 26501855; 29263897).