Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue, PC-3 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11474-1-AP targets UTP14A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2027 Product name: Recombinant human UTP14A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC014987 Sequence: MTANRLAESLLALSQQEELADLPKDYLLSESEDEGDNDGERKHQKLLEAISSLDGKNRRKLAERSEASLKVSEFNVSSEGSGEKLVLADLLEPVKTSSSLATVKKQLSRVKSKKTVELPLNKEEIERIHREVAFNKTAQVLSKWDPVVLKNRQAEQLVFPLEKEEPAIAPIEHVLSGWKARTPLEQEIFNLLHKNKQPVTDPLLTPVEKASLRAMSLEEAKMRRAELQRARALQSYYEAKARREKKIKSKKYHKVVKKGKAKKALKEFEQLRKVNPAAALEELEKIEKARMMERMSLKHQNSGKWAKSKAIMAKYDLEARQAMQEQLSKNKELTQKLQVASESEEEEGGT Predict reactive species |
| Full Name | UTP14, U3 small nucleolar ribonucleoprotein, homolog A (yeast) |
| Calculated Molecular Weight | 771 aa, 88 kDa |
| Observed Molecular Weight | 88 kDa |
| GenBank Accession Number | BC014987 |
| Gene Symbol | UTP14A |
| Gene ID (NCBI) | 10813 |
| RRID | AB_2272800 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BVJ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UTP14A antibody 11474-1-AP | Download protocol |
| WB protocol for UTP14A antibody 11474-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-UTP14A (Cat# 11474-1-AP) has 6 total citations published in Cell Reports, iScience, RNA, RNA Biology, Journal of Cancer, and Human Molecular Genetics. Research fields include ribosome biogenesis, RNA modification, protein ubiquitination, and cancer biology (colorectal and oesophageal cancers), with applications in WB and IF in human samples.
| Species | Application | Title |
|---|---|---|
Cell Rep UTP18-mediated p21 mRNA instability drives adenoma-carcinoma progression in colorectal cancer | ||
RNA NSUN6 is a human RNA methyltransferase that catalyzes formation of m5C72 in specific tRNAs. | ||
RNA Biol The DExD box ATPase DDX55 is recruited to domain IV of the 28S ribosomal RNA by its C-terminal region. | ||
J Cancer U three protein 14a (UTP14A) promotes tumour proliferation and metastasis via the PERK/eIF2a/GRP78 signalling pathway in oesophageal squamous cell carcinoma.
| ||
Hum Mol Genet Effects of the Bowen-Conradi syndrome mutation in EMG1 on its nuclear import, stability and nucleolar recruitment.
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