Tested Applications
| Positive WB detected in | K-562 cells, A375 cells, Jurkat cells |
| Positive IF/ICC detected in | A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11596-1-AP targets TFIIE Beta in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2136 Product name: Recombinant human TFIIE beta protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3-291 aa of BC030572 Sequence: PSLLRERELFKKRALSTPVVEKRSASSESSSSSSKKKKTKVEHGGSSGSKQNSDHSNGSFNLKALSGSSGYKFGVLAKIVNYMKTRHQRGDTHPLTLDEILDETQHLDIGLKQKQWLMTEALVNNPKIEVIDGKYAFKPKYNVRDKKALLRLLDQHDQRGLGGILLEDIEEALPNSQKAVKALGDQILFVNRPDKKKILFFNDKSCQFSVDEEFQKLWRSVTVDSMDEEKIEEYLKRQGISSMQESGPKKVAPIQRRKKPASQKKRRFKTHNEHLAGVLKDYSDITSSK Predict reactive species |
| Full Name | general transcription factor IIE, polypeptide 2, beta 34kDa |
| Calculated Molecular Weight | 291 aa, 33 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC030572 |
| Gene Symbol | GTF2E2 |
| Gene ID (NCBI) | 2961 |
| RRID | AB_2232722 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29084 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The general transcription factor IIE (TFIIE) is an essential component of the eukaryotic RNA polymerase II initiation complex, which plays pivotal roles in initiation of protein-coding gene transcription (PMID: 7829083). Transcription initiation factor IIE comprises two subunits, TFIIE-alpha and TFIIE-beta, two chains of which together form a tetramer. TFIIE-beta is encoded by GTF2E2 gene and contains TFIIE beta-DNA binding domain. It mainly functions to recruit TFIIH to the initiation complex and stimulate RNA polymerase II kinase activity (PMID: 10792464).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TFIIE Beta antibody 11596-1-AP | Download protocol |
| WB protocol for TFIIE Beta antibody 11596-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TFIIE Beta Polyclonal antibody (Cat# 11596-1-AP) has 2 total citations. These appear in Nucleic Acids Research and Cancer Gene Therapy. The cited research fields include stem cell biology and neuronal differentiation, as well as glioblastoma progression. Both citations used the antibody for Western blot applications, with one in mouse and one in human samples.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation. | ||
Cancer Gene Ther GTF2E2 downregulated by miR-340-5p inhibits the malignant progression of glioblastoma |
