FTSJ1 Polyclonal antibody

FTSJ1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11620-1-AP

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MRX44, JM23, FtsJ homolog 1 (E. coli), EC:2.1.1.205, CDLIV

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 11620-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11620-1-AP (FTSJ1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 11620-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11620-1-AP (FTSJ1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 11620-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11620-1-AP (FTSJ1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 11620-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11620-1-AP (FTSJ1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 11620-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11620-1-AP (FTSJ1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A431 using 11620-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using FTSJ1 antibody (11620-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, U-87 MG cells
Positive IHC detected in	human liver tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11620-1-AP targets FTSJ1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2199 Product name: Recombinant human FTSJ1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-329 aa of BC023584 Sequence: MGRTSKDKRDVYYRLAKENGWRARSAFKLLQLDKEFQLFQGVTRAVDLCAAPGSWSQVLSQKIGGQGSGHVVAVDLQAMAPLPGVVQIQGDITQLSTAKEIIQHFKGCPADLVVCDGAPDVTGLHDVDEYMQAQLLLAALNIATHVLKPGGCFVAKIFRGRDVTLLYSQLQVFFSSVLCAKPRSSRNSSIEAFAVCQGYDPPEGFIPDLSKPLLDHSYDPDFNQLDGPTRIIVPFVTCGDLSSYDSDRSYPLDLEGGSEYKYTPPTQPPISPPYQEACTLKRKGQLAKEIRPQDCPISRVDTFPQPLAAPQCHTLLAPEMEDNEMSCSP Predict reactive species
Full Name	FtsJ homolog 1 (E. coli)
Calculated Molecular Weight	329 aa, 36 kDa
Observed Molecular Weight	36 kDa
GenBank Accession Number	BC023584
Gene Symbol	FTSJ1
Gene ID (NCBI)	24140
RRID	AB_2877785
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UET6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for FTSJ1 antibody 11620-1-AP	Download protocol
WB protocol for FTSJ1 antibody 11620-1-AP	Download protocol
IF protocol for FTSJ1 antibody 11620-1-AP	Download protocol

Standard Protocols
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Publications

What published studies show

The FTSJ1 Polyclonal antibody (Cat# 11620-1-AP) has 3 total citations. These appear in Communications Biology, Biomolecules, and bioRxiv. The research fields span tRNA recognition and epitranscriptome biology, comparative testicular transcriptional and translational analysis in hybrid species, and Dengue virus exploitation of host tRNA modifications to promote viral replication.

Species	Application	Title
		Commun Biol Structural insights into tRNA recognition of the human FTSJ1-THADA complex Authors - Kensuke Ishiguro View Article
	WB	Biomolecules Comparative Analysis of Testicular Transcriptional and Translational Landscapes in Yak and Cattle-Yak: Implications for Hybrid Male Sterility Authors - Mengli Cao View Article
human	WB	bioRxiv Dengue virus exploits the host tRNA epitranscriptome to promote viral replication Authors - Cheryl Chan View Article