Tested Applications
| Positive WB detected in | human brain tissue, HeLa cells, HepG2 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11759-1-AP targets ZNF395 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2355 Product name: Recombinant human ZNF395 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 161-513 aa of BC001237 Sequence: DAVEMDEMMAAMVLTSLSCSPVVQSPPGTEANFSASRAACDPWKESGDISDSGSSTTSGHWSGSSGVSTPSPPHPQASPKYLGDAFGSPQTDHGFETDPDPFLLDEPAPRKRKNSVKVMYKCLWPNCGKVLRSIVGIKRHVKALHLGDTVDSDQFKREEDFYYTEVQLKEESAAAAAAAAAGTPVPGTPTSEPAPTPSMTGLPLSALPPPLHKAQSSGPEHPGPESSLPSGALSKSAPGSFWHIQADHAYQALPSFQIPVSPHIYTSVSWAAAPSAACSLSPVRSRSLSFSEPQQPAPAMKSHLIVTSPPRAQSGARKARGEAKKCRKVYGIEHRDQWCTACRWKKACQRFLD Predict reactive species |
| Full Name | zinc finger protein 395 |
| Calculated Molecular Weight | 513 aa, 55 kDa |
| Observed Molecular Weight | 62 kDa, 72 kDa |
| GenBank Accession Number | BC001237 |
| Gene Symbol | ZNF395 |
| Gene ID (NCBI) | 55893 |
| RRID | AB_2218065 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H8N7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZNF395 is a transcription factors binding to a 7bp GC rich sequence which resides in triplicate at intervals of 13bp within and proximal to the -20bp direct repeat sequences of the Htt promoter. It can bind to regulatory regions in papillomaviruses (PV) and subsequently called the protein papillomavirus binding factor (PBF) [PMID: 11853404]. It contains conserved regions CR1, CR2 and CR3, a domain rich in serines and prolines and have the potential to form a zinc-finger structure, and the C-terminal CR3 is responsible for DNA-binding [PMID: 17897615].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZNF395 antibody 11759-1-AP | Download protocol |
| IHC protocol for ZNF395 antibody 11759-1-AP | Download protocol |
| WB protocol for ZNF395 antibody 11759-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZNF395 Polyclonal antibody (Cat# 11759-1-AP) has 3 total citations. These publications appear in Journal of Experimental & Clinical Cancer Research, OncoTargets and Therapy, and Pathobiology. All three studies focus on oncology, investigating ZNF395's role in clear cell renal cell carcinoma, chondrosarcoma, and pancreatic ductal adenocarcinoma progression. The antibody was used for Western blotting and immunohistochemistry in human samples.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res HnRNP A1 - mediated alternative splicing of CCDC50 contributes to cancer progression of clear cell renal cell carcinoma via ZNF395.
| ||
Onco Targets Ther Dysregulation of Zinc Finger Protein 395 Contributes to the Pathogenesis of Chondrosarcoma. | ||
Pathobiology Downregulation of ZNF395 Drives Progression of Pancreatic Ductal Adenocarcinoma through Enhancement of Growth Potential. |
