Tested Applications
| Positive WB detected in | human kidney tissue, MCF-7 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11809-1-AP targets PSG1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2468 Product name: Recombinant human PSG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 35-349 aa of BC022338 Sequence: QVTIEAEPTKVSEGKDVLLLVHNLPQNLTGYIWYKGQMRDLYHYITSYVVDGEIIIYGPAYSGRETAYSNASLLIQNVTREDAGSYTLHIIKGDDGTRGVTGRFTFTLHLETPKPSISSSNLNPRETMEAVSLTCDPETPDASYLWWMNGQSLPMTHSLKLSETNRTLFLLGVTKYTAGPYECEIRNPVSASRSDPVTLNLLPKLPKPYITINNLNPRENKDVLNFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNETGPYQCEIRDRYGGIRSDPVTLNVLYGPDLPRIYPSFTYYRSGEV Predict reactive species |
| Full Name | pregnancy specific beta-1-glycoprotein 1 |
| Calculated Molecular Weight | 426 aa, 48 kDa |
| Observed Molecular Weight | 40-70 kDa |
| GenBank Accession Number | BC022338 |
| Gene Symbol | PSG1 |
| Gene ID (NCBI) | 5669 |
| RRID | AB_2171045 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11464 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Pregnancy-specific β1-glycoproteins (PSGs) are predominant proteins synthesized during gestation in humans, primates and rodents (PMID: 5001318; 11133662). In humans, PSGs are mainly secreted by syncytiotrophoblasts, while in rodents they derive from trophoblast giant cells and spongiotrophoblasts (PMID: 9290143). Moreover, PSGs can serve as indicators for blastocyst quality and embryonic viability (PMID: 8671387; 2409861).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PSG1 antibody 11809-1-AP | Download protocol |
| IHC protocol for PSG1 antibody 11809-1-AP | Download protocol |
| WB protocol for PSG1 antibody 11809-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PSG1 Polyclonal antibody (Cat# 11809-1-AP) has 2 citations. They appear in Fertility and Sterility (2014) and Journal of Obstetric Gynaecology Research (2025). The research fields encompass noninvasive prenatal screening via trophoblast isolation and vascular biology, specifically PSG1's role in regulating proliferation and migration of human umbilical vein endothelial cells through the TGF-β/Orai3 signaling pathway.
| Species | Application | Title |
|---|---|---|
Fertil Steril Trophoblast retrieval and isolation from the cervix (TRIC) for noninvasive prenatal screening at 5 to 20 weeks of gestation. | ||
J Obstet Gynaecol Res PSG1 in Regulating Proliferation and Migration of Human Umbilical Vein Endothelial Cells Through the TGF-β/Orai3 Signaling Pathway.
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