Tested Applications
| Positive WB detected in | human liver tissue, HepG2 cells, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue, human testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11823-1-AP targets CIB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2391 Product name: Recombinant human CIB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-191 aa of BC000846 Sequence: MGGSGSRLSKELLAEYQDLTFLTKQEILLAHRRFCELLPQEQRSVESSLRAQVPFEQILSLPELKANPFKERICRVFSTSPAKDSLSFEDFLDLLSVFSDTATPDIKSHYAFRIFDFDDDGTLNREDLSRLVNCLTGEGEDTRLSASEMKQLIDNILEESDIDRDGTINLSEFQHVISRSPDFASSFKIVL Predict reactive species |
| Full Name | calcium and integrin binding 1 (calmyrin) |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | BC000846 |
| Gene Symbol | CIB1 |
| Gene ID (NCBI) | 10519 |
| RRID | AB_2079679 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99828 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CIB1(Calcium and integrin-binding protein 1) is also named as CIB, KIP, PRKDCIP. It is an EF-hand-containing protein that binds multiple effector proteins, including the platelet αIIbβ3 integrin and several serine/threonine kinases and potentially modulates their function. It regulates platelet aggregation in hemostasis through a specific interaction with the alphaIIb cytoplasmic domain of platelet integrin alphaIIbbeta3.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CIB1 antibody 11823-1-AP | Download protocol |
| IHC protocol for CIB1 antibody 11823-1-AP | Download protocol |
| IP protocol for CIB1 antibody 11823-1-AP | Download protocol |
| WB protocol for CIB1 antibody 11823-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CIB1 Polyclonal antibody (Cat# 11823-1-AP) has 14 citations published in journals including Cell Death & Differentiation, Molecular Therapy Oncology, Frontiers in Immunology, Scientific Reports, PLoS Pathogens, Frontiers in Molecular Neuroscience, FASEB Journal, BMC Cancer, Journal of Virology, Journal of Cellular Physiology, F1000Research, Biology Direct, and Cell Death Discovery. Research fields span cancer biology, neuroscience, virology, liver physiology, pulmonary disease, and reproductive biology.
| Species | Application | Title |
|---|---|---|
Cell Death Differ CHIP-mediated CIB1 ubiquitination regulated epithelial-mesenchymal transition and tumor metastasis in lung adenocarcinoma. | ||
Mol Ther Oncol High CIB1 expression in colorectal cancer liver metastases correlates with worse survival and the replacement histopathological growth pattern | ||
Front Immunol Identification of calcium and integrin-binding protein 1 as a reprogrammed glucose metabolism mediator to restrict immune cell infiltration in the stromal compartment of pancreatic ductal adenocarcinoma
| ||
PLoS Pathog CIB1 Synergizes with EphrinA2 to Regulate Kaposi's Sarcoma-Associated Herpesvirus Macropinocytic Entry in Human Microvascular Dermal Endothelial Cells.
| ||
Front Mol Neurosci Expression of genes encoding the calcium signalosome in cellular and transgenic models of Huntington's disease. |
