Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11878-1-AP targets TTC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2474 Product name: Recombinant human TTC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-387 aa of BC001276 Sequence: MEQPGQDPTSDDVMDSFLEKFQSQPYRGGFHEDQWEKEFEKVPLFMTRAPSEIDPRENPDLACLQSIIFDEERSPEEQAKTYKDEGNDYFKEKDYKKAVISYTEGLKKKCADPDLNAVLYTNRAAAQYYLGNFRSALNDVTAARKLKPCHLKAIIRGALCHLELKHFAEAVNWCDEGLQIDAKEKKLLEMRAKADKLKRIEQRDVRKANLKEKKERNQNEALLQAIKARNIRLSEAACEDEDSASEGLGELFLDGLSTENPHGARLSLDGQGRLSWPVLFLYPEYAQSDFISAFHEDSRFIDHLMVMFGETPSWDLEQKYCPDNLEVYFEDEDRAELYRVPAKSTLLQVLQHQRYFVKALTPAFLVCVGSSPFCKNFLRGRKVYQIR Predict reactive species |
| Full Name | tetratricopeptide repeat domain 4 |
| Calculated Molecular Weight | 387 aa, 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC001276 |
| Gene Symbol | TTC4 |
| Gene ID (NCBI) | 7268 |
| RRID | AB_2211700 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95801 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TTC4 antibody 11878-1-AP | Download protocol |
| WB protocol for TTC4 antibody 11878-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this product (rated 4/5) describes use in Western Blot on K562 cells at a 1:1000 dilution. The antibody detected the target at the expected molecular weight and was validated by knockdown, confirming specificity. However, the reviewer noted an additional non-specific band appearing below the target band. Overall, the antibody performed well for confirming target expression but showed some background signal.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Coffee (Verified Customer) (03-20-2022) | Verified by knockdown at expected size, but there is an additional non-specific band underneath it.
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