Tested Applications
| Positive WB detected in | K-562 cells, HEK-293 cells, BxPC-3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11914-1-AP targets PLRG1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2527 Product name: Recombinant human PLRG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 203-505 aa of BC020786 Sequence: VEPGNQWFVTGSADRTIKIWDLASGKLKLSLTGHISTVRGVIVSTRSPYLFSCGEDKQVKCWDLEYNKVIRHYHGHLSAVYGLDLHPTIDVLVTCSRDSTARIWDVRTKASVHTLSGHTNAVATVRCQAAEPQIITGSHDTTIRLWDLVAGKTRVTLTNHKKSVRAVVLHPRHYTFASGSPDNIKQWKFPDGSFIQNLSGHNAIINTLTVNSDGVLVSGADNGTMHLWDWRTGYNFQRVHAAVQPGSLDSESGIFACAFDQSESRLLTAEADKTIKVYREDDTATEETHPVSWKPEIIKRKRF Predict reactive species |
| Full Name | pleiotropic regulator 1 (PRL1 homolog, Arabidopsis) |
| Calculated Molecular Weight | 514 aa, 57 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC020786 |
| Gene Symbol | PLRG1 |
| Gene ID (NCBI) | 5356 |
| RRID | AB_2165608 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43660 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PLRG1, identifed as a component of the CDC5L complex, is one spliceosomal protein highly conserved across species. It has been shown to be essential for pre-mRNA splicing in yeast and humans.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PLRG1 antibody 11914-1-AP | Download protocol |
| IP protocol for PLRG1 antibody 11914-1-AP | Download protocol |
| WB protocol for PLRG1 antibody 11914-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PLRG1 Polyclonal antibody (Cat# 11914-1-AP) has 2 total citations. These appear in Nucleic Acids Research and Cell Death & Differentiation. The cited research fields include RNA splicing and transcription regulation (intron retention, global transcription, cellular senescence) and tumor biology (nuclear speckle mRNA splicing, phase separation, and tumorigenesis). Both studies used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence. | ||
Cell Death Differ USP42 drives nuclear speckle mRNA splicing via directing dynamic phase separation to promote tumorigenesis. |
