Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11927-1-AP targets ERGIC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2603 Product name: Recombinant human ERGIC2 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-377 aa of BC000887 Sequence: MRRLNRKKTLSLVKELDAFPKVPESYVETSASGGTVSLIAFTTMALLTIMEFSVYQDTWMKYEYEVDKDFSSKLRINIDITVAMKCQYVGADVLDLAETMVASADGLVYEPTVFDLSPQQKEWQRMLQLIQSRLQEEHSLQDVIFKSAFKSTSTALPPREDDSSQSPNACRIHGHLYVNKVAGNFHITVGKAIPHPRGHAHLAALVNHESYNFSHRIDHLSFGELVPAIINPLDGTEKIAIDHNQMFQYFITVVPTKLHTYKISADTHQFSVTERERIINHAAGSHGVSGIFMKYDLSSLMVTVTEEHMPFWQFFVRLCGIVGGIFSTTGMLHGIGKFIVEIICCRFRLGSYKPVNSVPFEDGHTDNHLPLLENNTH Predict reactive species |
| Full Name | ERGIC and golgi 2 |
| Calculated Molecular Weight | 377 aa, 43 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC000887 |
| Gene Symbol | ERGIC2 |
| Gene ID (NCBI) | 51290 |
| RRID | AB_2098440 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RQ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ERGIC2, also named as Endoplasmic reticulum-Golgi intermediate compartment protein 2 or PTX1, is a 377 amino acid protein, which belongs to the ERGIC family. ERGIC2 is ubiquitously expressed in many tissues. ERGIC2 localizes in the endoplasmic reticulum-Golgi intermediate compartment membrane and may play a role in transport between endoplasmic reticulum and Golgi. ERGIC2 is involved in protein transportation, and participated in the elongation factor 1 alpha regulated mechanisms.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ERGIC2 antibody 11927-1-AP | Download protocol |
| IHC protocol for ERGIC2 antibody 11927-1-AP | Download protocol |
| IP protocol for ERGIC2 antibody 11927-1-AP | Download protocol |
| WB protocol for ERGIC2 antibody 11927-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ERGIC2 Polyclonal antibody (Cat# 11927-1-AP) has 2 citations. One appears in Molecular Biology of the Cell (2026), studying novel regulators of Sonic hedgehog secretion using proximity labeling and vesicle reconstitution assays. The other is in DNA and Cell Biology (2013), characterizing a variant of the ERGIC2 transcript. The research fields span cell biology, protein secretion pathways, and gene transcript analysis.
| Species | Application | Title |
|---|---|---|
Mol Biol Cell An integrated proximity labeling and vesicle reconstitution assay identifies novel regulators of Sonic hedgehog secretion.
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