Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, human brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11946-1-AP targets ATF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2548 Product name: Recombinant human ATF1 protein Source: e coli.-derived, T-GST Tag: GST Domain: 1-271 aa of BC029619 Sequence: MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSV Predict reactive species |
| Full Name | activating transcription factor 1 |
| Calculated Molecular Weight | 271 aa, 29 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC029619 |
| Gene Symbol | ATF1 |
| Gene ID (NCBI) | 466 |
| RRID | AB_2060777 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P18846 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TFs play a vital role in maintaining cell homeostasis by combining DNA regulatory sequences to regulate gene transcription rates. Activating transcription factor 1 (ATF1) is a member of the ATF/CREB family of transcription factors (TFs), specifically interacting with the consensus ATF/CRE site 'TGACGTCA'(PMID: 28032861). ATF1 transcriptionally regulates the expressions of target genes to mediate various cellular processes, and ATF1 effects the progression of tumors (including lung cancer, colorectal cancer,and nasopharyngeal carcinoma).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATF1 antibody 11946-1-AP | Download protocol |
| IP protocol for ATF1 antibody 11946-1-AP | Download protocol |
| WB protocol for ATF1 antibody 11946-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATF1 antibody (Cat# 11946-1-AP) has 12 total citations. These appear in journals including Signal Transduction and Targeted Therapy, Journal of Advanced Research, Cell Reports, American Journal of Human Genetics, Science Signaling, Molecular Therapy Nucleic Acids, Cells, Frontiers in Cell and Developmental Biology, Acta Biochimica et Biophysica Sinica, Journal of Biological Chemistry, Journal of Virology, and European Journal of Histochemistry. Research fields span esophageal cancer, colorectal cancer, lung adenocarcinoma, inflammation, neurogenesis, oxidative stress, mitochondrial function, viral replication, and cAMP signaling pathways.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Krüppel-like factor 4 downregulation during esophageal cancer formation facilitates immune evasion and impairs immunotherapy | ||
J Adv Res Transducin-like enhancer of split 3 protects against lipopolysaccharide-induced inflammation through DDX5-ATF1-PPP2R5A signaling | ||
Am J Hum Genet Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction.
| ||
Cell Rep LHPP-mediated inorganic pyrophosphate hydrolysis-driven lysosomal acidification in astrocytes regulates adult neurogenesis | ||
Mol Ther Nucleic Acids Mbd2 deficiency alleviates retinal cell apoptosisvia the miR-345-5p/Atf1 axis in high glucoseinjury and streptozotocin-induced diabetic mice. |
