Tested Applications
| Positive WB detected in | human testis tissue, HepG2 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11952-1-AP targets ASB9 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2554 Product name: Recombinant human ASB9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-252 aa of BC001244 Sequence: MDGKQGGMDGSKPAGPRDFPGIRLLSNPLMGDAVSDWSPMHEAAIHGHQLSLRNLISQGWAVNIITADHVSPLHEACLGGHLSCVKILLKHGAQVNGVTADWHTPLFNACVSGSWDCVNLLLQHGASVQPESDLASPIHEAARRAYGGNIDHKISHLGTPLYLACENQQRACVKKLLESGADVNQGKGQDSPLHAVARTASEELACLLMDFGADTQAKNAEGKRPVELVPPESPLAQLFLEREGASLPKPKP Predict reactive species |
| Full Name | ankyrin repeat and SOCS box-containing 9 |
| Calculated Molecular Weight | 252 aa, 28 kDa, 32 kDa |
| Observed Molecular Weight | 28-32 kDa |
| GenBank Accession Number | BC001244 |
| Gene Symbol | ASB9 |
| Gene ID (NCBI) | 140462 |
| RRID | AB_10666996 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96DX5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ASB9 antibody 11952-1-AP | Download protocol |
| WB protocol for ASB9 antibody 11952-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ASB9 Polyclonal antibody (Cat# 11952-1-AP) has 2 total citations. One appears in Molecules (2021), studying key candidate genes in idiopathic pulmonary fibrosis progression using IHC in human samples. The other is in Oncology Reports (2019), investigating how HMQ-T-F2 suppresses cervical cancer HeLa cell migration by reversing EMT via the PI3K/Akt signaling pathway. The associated research fields include pulmonary fibrosis, cancer biology, epithelial-mesenchymal transition, and signaling pathway analysis.
| Species | Application | Title |
|---|---|---|
Molecules Identification of Key Candidate Genes Involved in the Progression of Idiopathic Pulmonary Fibrosis. | ||
Oncol Rep HMQ‑T‑F2 suppresses migration of the human cervical cancer HeLa cells by reversing EMT via the PI3K/Akt signaling pathway. |
