Tested Applications
| Positive WB detected in | COLO 320 cells, HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human cervical cancer tissue, human stomach cancer tissue, mouse intestine, mouse thymus tissue, rat small intestine tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11975-1-AP targets FANCA in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2598 Product name: Recombinant human FANCA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-297 aa of BC008979 Sequence: MSDSWVPNSASGQDPGGRRRAWAELLAGRVKREKYNPERAQKLKESAVRLLRSHQDLNALLLEVEGPLCKKLSLSKVIDCDSSEAYANHSSSFIGSALQDQASRLGVPVGILSAGMVASSVGQICTAPAETSHPVLLTVEQRKKLSSLLEFAQYLLAHSMFSRLSFCQELWKIQSSLLLEAVWHLHVQGIVSLQELLESHPDMHAVGSWLFRNLCCLCEQMEASCQHADVARAMLSDFVQMFVLRGFQKNSDLRRTVEPEKMPQVAVDVLQRMLIFALDALAAGVQEESSTHKIVRC Predict reactive species |
| Full Name | Fanconi anemia, complementation group A |
| Calculated Molecular Weight | 163 kDa |
| Observed Molecular Weight | 163 kDa |
| GenBank Accession Number | BC008979 |
| Gene Symbol | FANCA |
| Gene ID (NCBI) | 2175 |
| RRID | AB_2101786 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15360 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FANCA, also named as FAA, FACA and FANCH, is a DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FANCA antibody 11975-1-AP | Download protocol |
| WB protocol for FANCA antibody 11975-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-FANCA antibody (Cat# 11975-1-AP) has 9 total citations published in journals including Nature Communications, Nature Structural & Molecular Biology, Journal of Translational Medicine, EMBO Molecular Medicine, Cell Reports, Acta Biochimica et Biophysica Sinica, Gene, and bioRxiv. Research fields span DNA damage repair, the Fanconi anemia pathway, replication stress response, cell cycle regulation, and cancer biology (hepatocellular carcinoma, lung adenocarcinoma, gastric cancer, esophageal atresia).
| Species | Application | Title |
|---|---|---|
Nat Commun Acetylation-dependent regulation of core spliceosome modulates hepatocellular carcinoma cassette exons and sensitivity to PARP inhibitors | ||
Nat Struct Mol Biol Fanconi anemia proteins participate in a break-induced-replication-like pathway to counter replication stress.
| ||
J Transl Med FANCA promotes lung adenocarcinoma progression and is a potential target for epitope vaccine immunotherapy | ||
Cell Rep C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair | ||
Acta Biochim Biophys Sin (Shanghai) FANCA facilitates G1/S cell cycle advancement, proliferation, migration and invasion in gastric cancer
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