Tested Applications
| Positive WB detected in | HeLa cells, U2OS cells, HEK-293 cells, A431 cells, PC-3 cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12038-1-AP targets HURP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2667 Product name: Recombinant human HURP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 498-847 aa of BC010658 Sequence: IKETTCTDLDGFWDMVSFQIEDVIHKFNNLIKLEESGWQVNNNMNHNMNKNVFRKKVVSGIASKPKQDDAGRIAARNRLAAIKNAMRERIRQEECAETAVSVIPKEVDKIVFDAGFFRVESPVKLFSGLSVSSEGPSQRLGTPKSVNKAVSQSRNEMGIPQQTTSPENAGPQNTKSEHVKKTLFLSIPESRSSIEDAQCPGLPDLIEENHVVNKTDLKVDCLSSERMSLPLLAGGVADDINTNKKEGISDVVEGMELNSSITSQDVLMSSPEKNTASQNSILEEGETKISQSELFDNKSLTTECHLLDSPGLNCSNPFTQLERRHQEHARHISFGGNLITFSPLQPGEF Predict reactive species |
| Full Name | discs, large (Drosophila) homolog-associated protein 5 |
| Calculated Molecular Weight | 846 aa, 95 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC010658 |
| Gene Symbol | HURP |
| Gene ID (NCBI) | 9787 |
| RRID | AB_10665363 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15398 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DLGAP5, also named as DAP 5 or DLG7, is a kinetochore protein that stabilizes microtubules in vicinity of chromosomes. DLG7 controls spindle dynamics, promotes interkinetochore tension and efficient kinetochore capture. DGL7 is a part of Ran-dependent complex. Expression of DGL7 is found in cancer and stem cells. DLGAP5 contributes to hepatocellular carcinoma tumorigenesis by promoting cell proliferation (PMID: 24324629).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HURP antibody 12038-1-AP | Download protocol |
| WB protocol for HURP antibody 12038-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-DLGAP5 antibody (Cat# 12038-1-AP) has 16 citations published in journals including Nature Communications, Journal of Translational Medicine, Life Sciences, Journal of Cell Biology, Cells, and others. Research fields span cancer biology (lung adenocarcinoma, prostate cancer, glioblastoma, hepatocellular carcinoma, pancreatic cancer), cell cycle and mitotic regulation, microtubule dynamics, and polycystic ovary syndrome. Applications include WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Nat Commun HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2 | ||
J Transl Med DLGAP5 promotes lung adenocarcinoma growth via upregulating PLK1 and serves as a therapeutic target
| ||
J Transl Med Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance | ||
Life Sci Pan-cancer analysis and experiments with cell lines reveal that the slightly elevated expression of DLGAP5 is involved in clear cell renal cell carcinoma progression.
| ||
Life Sci DLGAP5 upregulates E2F1 to promote prostate adenocarcinoma neuroendocrine differentiation | ||
J Cell Biol Diverse microtubule-binding repeats regulate TPX2 activities at distinct locations within the spindle.
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