CHN1 Polyclonal antibody

CHN1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12048-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

A chimaerin, Alpha chimerin, ARHGAP2, chimerin (chimaerin) 1, CHN, CHN1, DURS2, N chimaerin, N chimerin, NC, RHOGAP2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 12048-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12048-1-AP (CHN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human gliomas using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human gliomas using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human gliomas using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human gliomas using 12048-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12048-1-AP (CHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, PC-12 cells
Positive IHC detected in	human gliomas tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12048-1-AP targets CHN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2678 Product name: Recombinant human CHN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC011393 Sequence: MALTLFDTDEYRPPVWKSYLYQLQQEAPHPRRITCTCEVENRPKYYGREFHGMISREAADQLLIVAEGSYLIRESQRQPGTYTLALRFGSQTRNFRLYYDGKHFVGEKRFESIHDLVTDGLITLYIETKAAEYIAKMTINPIYEHVGYTTLNREPAYKKHMPVLKETHDERDSTGQDGVSEKRLTSLVRRATLKENEQIPKYEKIHNFKVHTFRGPHWCEYCANFMWGLIAQGVKCADCGLNVHKQCSKMVPNDCKPDLKHVKKVYSCDLTTLVKAHTTKRPMVVDMCIREIESRGLNSEGLYRVSGFSDLIEDVKMAFDRDGEKADISVNMYEDINIITGALKLYFRDL Predict reactive species
Full Name	chimerin (chimaerin) 1
Calculated Molecular Weight	459 aa, 53 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC011393
Gene Symbol	CHN1
Gene ID (NCBI)	1123
RRID	AB_10794446
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P15882
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for CHN1 antibody 12048-1-AP	Download protocol
WB protocol for CHN1 antibody 12048-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The CHN1 Polyclonal antibody (Cat# 12048-1-AP) has 2 citations. These publications appear in Bioengineered and BMC Cancer. The research fields covered are gastric cancer and cervical cancer, specifically examining the association of the CHN1 gene with gastric cancer progression and the role of miR-205-induced CHN1 upregulation in cervical cancer aggressiveness and lymph node metastasis. Both studies utilized WB and IHC applications in human samples.

Species	Application	Title
human	WB,IHC	Bioengineered Bioinformatics-based analysis of the association between the A1-chimaerin (CHN1) gene and gastric cancer Authors - Jie-Pin Li View Article
human	WB,IHC	BMC Cancer Upregulation of miR-205 induces CHN1 expression, which is associated with the aggressive behaviour of cervical cancer cells and correlated with lymph node metastasis. Authors - Jianbing Liu View Article