Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12159-1-AP targets GPRASP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2800 Product name: Recombinant human GPRASP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC013576 Sequence: MTGAEIEPSAQAKPEKKAGEEVIAGPERENDVPLVVRPKVRTQATTGARPKTETKSVPAARPKTEAQAMSGARPKTEVQVMGGARPKTEAQGITGARPKTDARAVGGARSKTDAKAIPGARPKDEAQAWAQSEFGTEAVSQAEGVSQTNAVAWPLATAESGSVTKSKGLSMDRELVNVDAETFPGTQGQKGIQPWFGPGEETNMGSWCYSRPRAREEASNESGFWSADETSTASSFWTGEETSVRSWPREESNTRSRHRAKHQTNPRSRPRSKQEAYVDSWSGSEDEASNPFSFWVGENTNNLFRPRVREEANIRSKLRTNREDCFESESEDEFYKQSWVLPGEEANSRFRH Predict reactive species |
| Full Name | G protein-coupled receptor associated sorting protein 2 |
| Calculated Molecular Weight | 838 aa, 94 kDa |
| Observed Molecular Weight | 94 kDa |
| GenBank Accession Number | BC013576 |
| Gene Symbol | GPRASP2 |
| Gene ID (NCBI) | 114928 |
| RRID | AB_2232653 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96D09 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPRASP2 antibody 12159-1-AP | Download protocol |
| WB protocol for GPRASP2 antibody 12159-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-GPRASP2 antibody (Cat# 12159-1-AP) has 4 citations published in Nature Communications, Communications Biology, Scientific Reports, and Journal of Medical Genetics. The associated research fields include GPRASP2-mediated synaptic plasticity and autism-like behaviors, iron homeostasis and cochlear hair cell function in hearing loss, maternal behavior and neurodevelopmental effects of Gprasp2 deletion, and X-linked recessive syndromic hearing loss genetics. Applications span WB, IF, and IHC in mouse models.
| Species | Application | Title |
|---|---|---|
Nat Commun Abnormal mGluR-mediated synaptic plasticity and autism-like behaviours in Gprasp2 mutant mice.
| ||
Commun Biol Abnormal iron homeostasis mediates cochlear hair cell impairment and hearing loss in Gprasp2-deficient mice.
| ||
Sci Rep Maternal behaviours disrupted by Gprasp2 deletion modulate neurodevelopmental trajectory in progeny.
| ||
J Med Genet GPRASP2, a novel causative gene mutated in an X-linked recessive syndromic hearing loss. |
Reviews
What customers say
The product received a 4-star rating from a researcher who used it at a 1:500 dilution for Western Blot on mouse tissue and hippocampal rat culture. No additional written comments were provided. Overall, the review suggests satisfactory performance for WB applications across both mouse and rat samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mohamed (Verified Customer) (08-07-2018) |
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