Tested Applications
| Positive WB detected in | HeLa cells, A375 cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12238-1-AP targets MKRN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2881 Product name: Recombinant human MKRN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 67-416 aa of BC015715 Sequence: GTMAHSVPSPAFHSPHPPSEVTASIVKTNSHEPGKREKRTLVLRDRNLSGMAERKTQPSMVSNPGSCSDPQPSPEMKPHSYLDAIRSGLDDVEASSSYSNEQQLCPYAAAGECRFGDACVYLHGEVCEICRLQVLHPFDPEQRKAHEKICMLTFEHEMEKAFAFQASQDKVCSICMEVILEKASASERRFGILSNCNHTYCLSCIRQWRCAKQFENPIIKSCPECRVISEFVIPSVYWVEDQNKKNELIEAFKQGMGKKACKYFEQGKGTCPFGSKCLYRHAYPDGRLAEPEKPRKQLSSQGTVRFFNSVRLWDFIENRESRHVPNNEDVDMTELGDLFMHLSGVESSEP Predict reactive species |
| Full Name | makorin ring finger protein 2 |
| Calculated Molecular Weight | 416 aa, 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC015715 |
| Gene Symbol | MKRN2 |
| Gene ID (NCBI) | 23609 |
| RRID | AB_2142636 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H000 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MKRN2(Probable E3 ubiquitin-protein ligase makorin-2) is also named as RNF62. It is an E3 ubiquitin ligase that catalyzes the covalent attachment of ubiquitin moieties onto substrate proteins. It may be playing a functional role in negatively regulating neurogenesis via PI3K/Akt signaling.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MKRN2 antibody 12238-1-AP | Download protocol |
| IHC protocol for MKRN2 antibody 12238-1-AP | Download protocol |
| IP protocol for MKRN2 antibody 12238-1-AP | Download protocol |
| WB protocol for MKRN2 antibody 12238-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MKRN2 Polyclonal antibody (Cat# 12238-1-AP) has 10 citations published in journals including Nature Immunology, Molecular Cell, Journal of Investigative Dermatology, International Immunopharmacology, Cancers, Human Cell, Biochemical and Biophysical Research Communications, Oncology Letters, and Cancer Biomarkers. Research fields span E3 ubiquitin ligase function, inflammation, autophagy, atherosclerosis, and multiple cancer types including bladder cancer, hepatocellular carcinoma, neuroblastoma, melanoma, and renal cell carcinoma.
| Species | Application | Title |
|---|---|---|
Nat Immunol The RNA-binding E3 ligase MKRN2 selectively disrupts Il6 translation to restrain inflammation | ||
Mol Cell Global profiling of nascent chain interactors reveals TRIM25 as a co-translational E3 ubiquitin ligase.
| ||
J Invest Dermatol N6-methyladenosine modification of lnc-CCKAR-5 regulates autophagy in human umbilical cord mesenchymal stem cells by destabilizing LMNA and inhibits diabetic wound healing | ||
Int Immunopharmacol Poliumoside attenuates endothelial senescence and atherosclerosis by regulating MKRN2-mediated autophagy via the PI3K/AKT/ mTOR pathway. | ||
Cancers (Basel) MKRN2-Mediated Degradation of IGF2BP3 Suppresses MYC and Enhances CDK4/6 Inhibitor Sensitivity in Bladder Cancer. | ||
Hum Cell MKRN2 enhances hepatocellular carcinoma proliferation through the p38 MAPK pathway.
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