Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human stomach cancer tissue, mouse testis tissue, mouse stomach tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12258-1-AP targets TBPL1 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2880 Product name: Recombinant human TBPL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-186 aa of BC017559 Sequence: MDADSDVALDILITNVVCVFRTRCHLNLRKIALEGANVIYKRDVGKVLMKLRKPRITATIWSSGKIICTGATSEEEAKFGARRLARSLQKLGFQVIFTDFKVVNVLAVCNMPFEIRLPEFTKNNRPHASYEPELHPAVCYRIKSLRATLQIFSTGSITVTGPNVKAVATAVEQIYPFVFESRKEIL Predict reactive species |
| Full Name | TBP-like 1 |
| Calculated Molecular Weight | 186 aa, 21 kDa |
| Observed Molecular Weight | 21 kDa, 25 kDa |
| GenBank Accession Number | BC017559 |
| Gene Symbol | TBPL1 |
| Gene ID (NCBI) | 9519 |
| RRID | AB_10642841 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62380 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for TBPL1 antibody 12258-1-AP | Download protocol |
| WB protocol for TBPL1 antibody 12258-1-AP | Download protocol |
| IHC protocol for TBPL1 antibody 12258-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TBPL1 polyclonal antibody (Cat# 12258-1-AP) has 4 citations published in Cell, Nature Communications, Pharmacological Research, and Cell Signaling. Research fields span reproductive biology (mouse spermiogenesis), molecular biology (rRNA biogenesis and nucleolar Pol II interactome), and pulmonary disease (bleomycin-induced lung fibrosis via cGAS-STING pathway, and idiopathic pulmonary fibrosis via C-MYC/miR-9-5p regulation). Cited applications include WB, IF, IP, and ChIP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesis | ||
Pharmacol Res Fluvoxamine alleviates bleomycin-induced lung fibrosis via regulating the cGAS-STING pathway | ||
Cell Signal C-MYC induces idiopathic pulmonary fibrosis via modulation of miR-9-5p-mediated TBPL1. |
Reviews
What customers say
The single review for 12258-1-AP (rated 4/5) reports strong recognition of human TBPL1 in cell culture lysate by Western Blot at a 1:1000 dilution, producing a nice clear band. However, the reviewer notes that efficiency against zebrafish TBPL1 is very low. Overall, the antibody performs well for human samples but shows limited cross-reactivity with zebrafish.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jianing (Verified Customer) (09-03-2019) | This antibody recognize human tbpl1 strongly which give a nice clear band. But the efficiency against zebrafish tbpl1 is very low.
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