Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, mouse brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue, human breast cancer tissue, human normal colon, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12345-1-AP targets DYNC1H1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2999 Product name: Recombinant human KIAA0325 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 590-989 aa of BC021297 Sequence: MAQSIYGGRVDNEFDQRLLNTFLERLFTTRSFDSEFKLACKVDGHKDIQMPDGIRREEFVQWVELLPDTQTPSWLGLPNNAERVLLTTQGVDMISKMLKMQMLEDEDDLAYAETEKKTRTDSTSDGRPAWMRTLHTTASNWLHLIPQTLSHLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTLINELVKGILPRSWSHYTVPAGMTVIQWVSDFSERIKQLQNISLAAASGGAKELKNIHVCLGGLFVPEAYITATRQYVAQANSWSLEELCLEVNVTTSQGATLDACSFGVTGLKLQGATCNNNKLSLSNAISTALPLTQLRWVKQTNTEKKASVVTLPVYLNFTRADLIFTVDFEIATKEDPRSFYERGVAVLCTE Predict reactive species |
| Full Name | dynein, cytoplasmic 1, heavy chain 1 |
| Calculated Molecular Weight | 4646 aa, 532 kDa |
| Observed Molecular Weight | 532 kDa |
| GenBank Accession Number | BC021297 |
| Gene Symbol | DYNC1H1 |
| Gene ID (NCBI) | 1778 |
| RRID | AB_2261765 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14204 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Dyneins are a group of microtubule-activated ATPases that convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein comprises 2 heavy chain polypeptides and several intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DYNC1H1 antibody 12345-1-AP | Download protocol |
| IHC protocol for DYNC1H1 antibody 12345-1-AP | Download protocol |
| IP protocol for DYNC1H1 antibody 12345-1-AP | Download protocol |
| WB protocol for DYNC1H1 antibody 12345-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
12345-1-AP is a DYNC1H1 polyclonal antibody with 74 citations. Publications appear in high-impact journals including Nature, Science, Neuron, Nature Cell Biology, Nature Communications, EMBO Journal, Journal of Cell Biology, Cell Reports, and Journal of Cell Science. Research fields span cytoskeletal and microtubule biology, dynein motor function, axonal transport, spindle assembly, autophagy, endocytosis, neuroscience, photoreceptor development, cancer biology, ciliogenesis, and viral trafficking.
| Species | Application | Title |
|---|---|---|
Nature Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.
| ||
Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. | ||
Nat Cell Biol Recycling of autophagosomal components from autolysosomes by the recycler complex. |
Reviews
What customers say
The single review for this product is highly positive, rated 5 out of 5 stars. The reviewer used the antibody for Western Blot and reported a clear, clean signal at the expected molecular weight, describing it as a very good antibody overall.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (08-24-2021) | Very good antibody, giving a clear and clean signal at the expected molecular weight.
|
