Tested Applications
| Positive WB detected in | mouse liver tissue, HepG2 cells, mouse colon tissue, mouse pancreas tissue |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12370-1-AP targets TSKU in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3054 Product name: Recombinant human TSKU protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC020975 Sequence: MPWPLLLLLAVSGAQTTRPCFPGCQCEVETFGLFDSFSLTRVDCSGLGPHIMPVPIPLDTAHLDLSSNRLEMVNESVLAGPGYTTLAGLDLSHNLLTSISPTAFSRLRYLESLDLSHNGLTALPAESFTSSPLSDVNLSHNQLREVSVSAFTTHSQGRALHVDLSHNLIHRLVPHPTRAGLPAPTIQSLNLAWNRLHAVPNLRDLPLRYLSLDGNPLAVIGPGAFAGLGGLTHLSLASLQRLPELAPSGFRELPGLQVLDLSGNPKLNWAGAEVFSGLSSLQELDLSGTNLVPLPEALLLHLPALQSVSVGQDVRCRRLVREGTYPRRPGSSPKVALHCVDTRDSAARGPTIL Predict reactive species |
| Full Name | tsukushin |
| Calculated Molecular Weight | 353 aa, 38 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC020975 |
| Gene Symbol | TSKU |
| Gene ID (NCBI) | 25987 |
| RRID | AB_2877851 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WUA8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TSK, also known as Tsukushi, is an atypical member of the small leucine-rich proteoglycan (SLRP) family of extracellular matrix proteins (PMID: 16884925). The human TSK cDNA encodes a 16 amino acid (aa) signal sequence and a 337 aa secreted protein that contains nine LRR, which has a calculated molecular mass of 38kDa and an apparent molecular mass of 40-50kDa cause of the N-glycosylation. In chick embryos, C-TSK is a unique BMP inhibitor that forms a ternary complex with BMP and chordin and is involved in the regulation of primitive streak and node formation. C-TSK migrates as a band of 47 kDa in SDS-PAGE and N-glycosidase F treatment reduces the size to 41 kDa (PMID: 15363410).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TSKU antibody 12370-1-AP | Download protocol |
| WB protocol for TSKU antibody 12370-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TSKU antibody (Cat# 12370-1-AP) has 1 citation. It was used in a study published in Cell Communication and Signaling (IF 11.6), investigating how SMYD3 synergizes with RACK1 to promote colorectal cancer lung metastasis by recruiting SMAD3. The antibody was applied in WB, IHC, and IF on human samples. The research falls within the fields of cancer biology, specifically colorectal cancer metastasis and TGF-β/SMAD signaling pathways.
| Species | Application | Title |
|---|---|---|
Cell Commun Signal SMYD3 synergises with RACK1 to promote colorectal cancer lung metastasis by recruiting SMAD3. |
