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NDUFS1 Polyclonal antibody

NDUFS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12444-1-AP

Publications (127)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial, EC:7.1.1.2, Complex I-75kD, Complex I 75kD, CI-75kD

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHCIF/ICC

WB analysis using 12444-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 12444-1-AP
WB result of NDUFS1 antibody (12444-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFS1 transfected HEK-293 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 12444-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse liver using 12444-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 12444-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 12444-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A549 using 12444-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12444-1-AP (NDUFS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse lung using 12444-1-AP
IP result of anti-NDUFS1 (IP:12444-1-AP, 4ug; Detection:12444-1-AP 1:500) with mouse lung tissue lysate 2800ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 12444-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:200 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 12444-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 12444-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 12444-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12444-1-AP (NDUFS1 antibody) at dilution of 1:200 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 12444-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS1 antibody (12444-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 12444-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NDUFS1 antibody (12444-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (127)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse kidney tissue, mouse liver tissue, HEK-293 cells, A549 cells, HeLa cells, HEK-293 tissue, rat kidney tissue
Positive IP detected in	mouse lung tissue
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12444-1-AP targets NDUFS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, drosophila
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3135 Product name: Recombinant human NDUFS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-305 aa of BC030833 Sequence: MLRIPVRKALVGLSKSPKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGNDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTAQPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEP Predict reactive species
Full Name	NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
Calculated Molecular Weight	727 aa, 79 kDa
Observed Molecular Weight	74-81 kDa
GenBank Accession Number	BC030833
Gene Symbol	NDUFS1
Gene ID (NCBI)	4719
RRID	AB_2282657
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P28331
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.

Protocols

Product Specific Protocols
IF protocol for NDUFS1 antibody 12444-1-AP	Download protocol
IHC protocol for NDUFS1 antibody 12444-1-AP	Download protocol
IP protocol for NDUFS1 antibody 12444-1-AP	Download protocol
WB protocol for NDUFS1 antibody 12444-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

NDUFS1 Polyclonal antibody (Cat# 12444-1-AP) has accumulated 127 citations across more than 80 journals, including Nature, Science, Nature Medicine, Cell Reports, Molecular Cell, Cell Research, Cancer Discovery, and EMBO Journal. The cited research spans diverse fields: mitochondrial complex I biology and oxidative phosphorylation, cardiovascular disease (ischemia-reperfusion injury), oncology (colorectal, pancreatic, breast, lung, renal cancers), neurodegeneration (Parkinson's, Alzheimer's, ALS, Friedreich's ataxia), ferroptosis, metabolic reprogramming, diabetes, and renal pathology.

Species	Application	Title
rat	WB	Nature Mitochondrial dysfunction abrogates dietary lipid processing in enterocytes Authors - Chrysanthi Moschandrea View Article
mouse	WB	Nat Med Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I. Authors - Chouchani Edward T ET View Article
human	WB	Science Autoregulatory control of mitochondrial glutathione homeostasis Authors - Yuyang Liu View Article
mouse	WB	Cell Metab Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals Authors - Timothy C Kenny View Article
human	WB	Cell Res MFSD7c functions as a transporter of choline at the blood-brain barrier Authors - Xuan Thi Anh Nguyen View Article
mouse	WB	Cell Res NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. Authors - Tingting Hou View Article