Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, human brain tissue, Jurkat cells, mouse brain tissue, mouse kidney tissue, mouse placenta tissue |
| Positive IP detected in | HeLa cells, mouse kidney tissue |
| Positive IHC detected in | human colon tissue, human kidney tissue, human normal colon, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12542-1-AP targets Annexin VI in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3263 Product name: Recombinant human ANXA6 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 324-674 aa of BC017046 Sequence: DDDAAGQFFPEAAQVAYQMWELSAVARVELKGTVRPANDFNPDADAKALRKAMKGLGTDEDTIIDIITHRSNVQRQQIRQTFKSHFGRDLMTDLKSEISGDLARLILGLMMPPAHYDAKQLKKAMEGAGTDEKALIEILATRTNAEIRAINEAYKEDYHKSLEDALSSDTSGHFRRILISLATGHREEGGENLDQAREDAQVAAEILEIADTPSGDKTSLETRFMTILCTRSYPHLRRVFQEFIKMTNYDVEHTIKKEMSGDVRDAFVAIVQSVKNKPLFFADKLYKSMKGAGTDEKTLTRIMVSRSEIDLLNIRREFIEKYDKSLHQAIEGDTSGDFLKALLALCGGED Predict reactive species |
| Full Name | annexin A6 |
| Calculated Molecular Weight | 673 aa, 76 kDa |
| Observed Molecular Weight | 67-72 kDa |
| GenBank Accession Number | BC017046 |
| Gene Symbol | Annexin VI |
| Gene ID (NCBI) | 309 |
| RRID | AB_2057753 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08133 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Annexin VI antibody 12542-1-AP | Download protocol |
| IHC protocol for Annexin VI antibody 12542-1-AP | Download protocol |
| IP protocol for Annexin VI antibody 12542-1-AP | Download protocol |
| WB protocol for Annexin VI antibody 12542-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Annexin A6 antibody (Cat# 12542-1-AP) has 16 citations spanning journals including J Extracell Vesicles, Adv Sci, Cell Death Dis, CNS Neurosci Ther, Life Sci, Matrix Biol, Int J Mol Sci, Oncotarget, Front Cell Dev Biol, J Proteome Res, Proteome Sci, Anat Rec, AIMS Microbiol, and Onco Targets Ther. Research fields cover extracellular vesicles, cancer (breast, bladder, leukemia, liver, retinoblastoma), neurology, cardiovascular disease, bone biology, autophagy, proteomics, and microbiology.
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion-exchange method. | ||
Adv Sci (Weinh) Isolation and Comprehensive Analysis of Cochlear Tissue-Derived Small Extracellular Vesicles | ||
Adv Sci (Weinh) Small Extracellular Vesicles Orchestrate Cisplatin-Induced Ototoxicity: Potential Biomarker and Targets Discovery | ||
Cell Death Dis Exosomal annexin A6 induces gemcitabine resistance by inhibiting ubiquitination and degradation of EGFR in triple-negative breast cancer. | ||
CNS Neurosci Ther Annexin A6 mitigates neurological deficit in ischemia/reperfusion injury by promoting synaptic plasticity | ||
Life Sci Transcription factor PRRX1-activated ANXA6 facilitates EGFR-PKCα complex formation and enhances cisplatin sensitivity in bladder cancer
|
Reviews
What customers say
Both reviewers gave 5-star ratings. The antibody performs reliably in Western Blot, Immunoprecipitation, and cell culture applications. It produces the expected band at the correct molecular weight and works across multiple cell types, including HEK293, MOVAS, A7R5, smooth muscle cells, aorta, and placental trophoblasts. Reviewers confirmed compatibility with various blocking conditions, including TBST, 5% skimmed milk, and 3% BSA in TBST. Recommended dilutions range from 1:2000 to 1:5000 depending on the application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Gaurav (Verified Customer) (02-27-2026) | Works with different types of cells
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Gaurav (Verified Customer) (02-29-2024) | Very good antibody with expected band at appropriate position. Works well in TBST, 5% Skimmed milk and 3% BSA in TBST
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