Tested Applications
| Positive WB detected in | HepG2 cells, K-562 cells, mouse heart tissue, human heart tissue, L02 cells, human brain tissue |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lung cancer tissue, human ovary cancer tissue, human placenta tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12586-1-AP targets SAMHD1 in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3287 Product name: Recombinant human SAMHD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-315 aa of BC036450 Sequence: MQRADSEQPSKRPRCDDSPRTPSNTPSAEADWSPGLELHPDYKTWGPEQVCSFLRRGGFEEPVLLKNIRENEITGALLPCLDESRFENLGVSSLGERKKLLSYIQRLVQIHVDTMKVINDPIHGHIELHPLLVRIIDTPQFQRLRYIKQLGGGYYVFPGASHNRFEHSLGVGYLAGCLVHALGEKQPELQISERDVLCVQIAGLCHDLGHGPFSHMFDGRFIPLARPEVKWTHEQGSVMMFEHLINSNGIKPVMEQYGLIPEEDICFIKEQIVGPLESPVEDSLWPYKGRPENKSFLYEIVSNKRNGIDVDKWDY Predict reactive species |
| Full Name | SAM domain and HD domain 1 |
| Calculated Molecular Weight | 626 aa, 72 kDa |
| Observed Molecular Weight | 64-72 kDa |
| GenBank Accession Number | BC036450 |
| Gene Symbol | SAMHD1 |
| Gene ID (NCBI) | 25939 |
| RRID | AB_2183496 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3Z3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for SAMHD1 antibody 12586-1-AP | Download protocol |
| IF protocol for SAMHD1 antibody 12586-1-AP | Download protocol |
| IHC protocol for SAMHD1 antibody 12586-1-AP | Download protocol |
| IP protocol for SAMHD1 antibody 12586-1-AP | Download protocol |
| WB protocol for SAMHD1 antibody 12586-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-SAMHD1 antibody (Cat# 12586-1-AP) has 88 total citations published in journals including Nature Medicine, Science, Immunity, Nature Genetics, Nature Communications, PNAS, EMBO Journal, Cell Reports, and J Virol. Associated research fields span virology (HIV, HBV, HCMV), oncology (AML, breast cancer, ovarian cancer, lymphoma), innate immunity, autoinflammatory diseases (Aicardi-Goutières syndrome), and inflammatory signaling pathways.
| Species | Application | Title |
|---|---|---|
Science Nucleotide metabolic rewiring enables NLRP3 inflammasome hyperactivation in obesity.
| ||
Immunity Tissue Myeloid Cells in SIV-Infected Primates Acquire Viral DNA through Phagocytosis of Infected T Cells. | ||
Nat Genet Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as regulator of the innate immune response. |
Reviews
What customers say
One reviewer (Roy Matkovic, June 2024) gave this antibody a 5-star rating for Western Blot. Using a 1/1000 dilution, they confirmed specific recognition of SAMHD1 in THP1 and MDM cells, with validation through HIV-2 Vpx treatment. Overall, the feedback highlights strong specificity and reliable performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Roy (Verified Customer) (06-15-2024) | Great antibody that recognizes specifically SAMHD1 in THP1 cells and MDM, validated through the use of HIV-2 Vpx
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