Tested Applications
| Positive WB detected in | mouse pancreas tissue, rat pancreas tissue |
| Positive IHC detected in | human pancreas tissue, human kidney tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12600-1-AP targets CPB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3243 Product name: Recombinant human PASP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 73-417 aa of BC015338 Sequence: AEDTVTVENVLKQNELQYKVLISNLRNVVEAQFDSRVRATGHSYEKYNKWETIEAWTQQVATENPALISRSVIGTTFEGRAIYLLKVGKAGQNKPAIFMDCGFHAREWISPAFCQWFVREAVRTYGREIQVTELLDKLDFYVLPVLNIDGYIYTWTKSRFWRKTRSTHTGSSCIGTDPNRNFDAGWCEIGASRNPCDETYCGPAAESEKETKALADFIRNKLSSIKAYLTIHSYSQMMIYPYSYAYKLGENNAELNALAKATVKELASLHGTKYTYGPGATTIYPAAGGSDDWAYDQGIRYSFTFELRDTGRYGFLLPESQIRATCEETFLAIKYVASYVLEHLY Predict reactive species |
| Full Name | carboxypeptidase B1 (tissue) |
| Calculated Molecular Weight | 417 aa, 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC015338 |
| Gene Symbol | CPB1 |
| Gene ID (NCBI) | 1360 |
| RRID | AB_2261100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15086 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PASP(pancreas-specific protein) is also named as CPB1, CPB, PCPB and belongs to the peptidase M14 family. This protein modulates the plasminogen system both in the matrix and on the cell surface, thus leading to the inhibition of endothelial cell movement and tube formation. It is initially described as a pancreatic metallocarboxypeptidase and is a marker for acute pancreatitis(PMID:19717511). The deduced 416-amino acid preproenzyme has a 15-amino acid signal peptide and a 95-amino acid activation peptide.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CPB1 antibody 12600-1-AP | Download protocol |
| WB protocol for CPB1 antibody 12600-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Carboxypeptidase B1 (CPB1) antibody (Cat# 12600-1-AP) has 4 total citations. These appear in Translational Oncology, Cancers (Basel), Journal of Bioenergetics and Biomembranes, and Archives of Gynecology and Obstetrics. Research fields include pancreatic ductal adenocarcinoma biomarker discovery, ductal carcinoma in situ prognosis, lens epithelial cell apoptosis and oxidative stress regulation, and menopausal osteoporosis signaling. Applications span WB and IHC in human and rat samples.
| Species | Application | Title |
|---|---|---|
Transl Oncol Label-Free Quantitative Proteomics Unravels Carboxypeptidases as the Novel Biomarker in Pancreatic Ductal Adenocarcinoma. | ||
J Bioenerg Biomembr METTL3 mediates CPB1 expression by regulating transcription factor BACH2 to promote apoptosis and oxidative stress of lens epithelial cells | ||
Arch Gynecol Obstet Oviductus Ranae protein hydrolyzate prevents menopausal osteoporosis by regulating TGFβ/BMP2 signaling. |
