Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12603-1-AP targets IPPK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3278 Product name: Recombinant human IPPK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 192-491 aa of BC026154 Sequence: RMHFALKSLLQEAQNNLKIFKNGELIYGCKDARSPVADWSELAHHLKPFFFPSNGLASGPHCTRAVIRELVHVITRVLLSGSDKGRAGTLSPGLGPQGPRVCEASPFSRSLRCQGKNTPERSGLPKGCLLYKTLQVQMLDLLDIEGLYPLYNRVERYLEEFPEERKTLQIDGPYDEAFYQKLLDLSTEDDGTVAFALTKVQQYRVAMTAKDCSIMIALSPCLQDASSDQRPVVPSSRSRFAFSVSVLDLDLKPYESIPHQYKLDGKIVNYYSKTVRAKDNAVMSTRFKESEDCTLVLHKV Predict reactive species |
| Full Name | inositol 1,3,4,5,6-pentakisphosphate 2-kinase |
| Calculated Molecular Weight | 491 aa, 56 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC026154 |
| Gene Symbol | IPPK |
| Gene ID (NCBI) | 64768 |
| RRID | AB_2127848 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H8X2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IPPK antibody 12603-1-AP | Download protocol |
| WB protocol for IPPK antibody 12603-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IPPK polyclonal antibody (Cat# 12603-1-AP) has 1 citation. It was published in Proceedings of the National Academy of Sciences of the United States of America (PNAS, IF 9.5) in 2016. The study investigated how inositol hexakisphosphate generated by IP5K mediates cullin-COP9 signalosome interactions and CRL function, placing this citation in the fields of cell signaling and ubiquitin-proteasome pathway regulation. The antibody was used for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Inositol hexakisphosphate (IP6) generated by IP5K mediates cullin-COP9 signalosome interactions and CRL function.
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