Tested Applications
| Positive WB detected in | human heart tissue, human brain tissue, human placenta tissue, human liver tissue, mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12630-1-AP targets PLSCR4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3323 Product name: Recombinant human PLSCR4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-272 aa of BC028354 Sequence: MSGVVPTAPEQPAGEMENQTKPPDPRPDAPPEYNSHFLPGPPGTAVPPPTGYPGGLPMGYYSPQQPSTFPLYQPVGGIHPVRYQPGKYPMPNQSVPITWMPGPTPMANCPPGLEYLVQLDNIHVLQHFEPLEMMTCFETNNRYDIKNNSDQMVYIVTEDTDDFTRNAYRTLRPFVLRVTDCMGREIMTMQRPFRCTCCCFCCPSARQELEVQCPPGVTIGFVAEHWNLCRAVYSIQNEKKENVMRVRGPCSTYGCGSDSVFEVKSLDGISNI Predict reactive species |
| Full Name | phospholipid scramblase 4 |
| Calculated Molecular Weight | 329 aa, 37 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC028354 |
| Gene Symbol | PLSCR4 |
| Gene ID (NCBI) | 57088 |
| RRID | AB_2165948 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRQ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PLSCR4 (Phospholipid scramblase 4) belongs to the phospholipid scramblase family which is thought to mediate the loss of lipid asymmetry. Elimination of this lipid bilayer asymmetry is an important physiological process and appears to occur as a result of elevated intracellular calcium concentration, or occur with cell activation, injury, and apoptosis.PLSCR4 also plays a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLSCR4 antibody 12630-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PLSCR4 Polyclonal antibody (Cat# 12630-1-AP) has 2 total citations. These appear in J Biochem Mol Toxicol and Reprod Sci. The cited research spans two fields: one study investigated how loss of TRAIP attenuates breast cancer cell development by regulating PLSCR4 stabilization (human, WB), while the other examined phospholipid scramblase expression in the pregnant mouse uterus during LPS-induced preterm delivery (mouse, WB).
| Species | Application | Title |
|---|---|---|
J Biochem Mol Toxicol Loss of TRAIP Could Attenuate the Breast Cancer Cells Development by Regulating PLSCR4 Stabilization.
| ||
Reprod Sci Phospholipid scramblase expression in the pregnant mouse uterus in LPS-induced preterm delivery. |
