Tested Applications
| Positive WB detected in | human brain tissue, HEK-293 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12643-1-AP targets ENPP6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3339 Product name: Recombinant human ENPP6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 90-440 aa of BC035035 Sequence: IGNYMWDPTTNKSFDIGVNKDSLMPLWWNGSEPLWVTLTKAKRKVYMYYWPGCEVEILGVRPTYCLEYKNVPTDINFANAVSDALDSFKSGRADLAAIYHERIDVEGHHYGPASPQRKDALKAVDTVLKYMTKWIQERGLQDRLNVIIFSDHGMTDIFWMDKVIELNKYISLNDLQQVKDRGPVVSLWPAPGKHSEIYNKLSTVEHMTVYEKEAIPSRFYYKKGKFVSPLTLVADEGWFITENREMLPFWMNSTGRREGWQRGWHGYDNELMDMRGIFLAFGPDFKSNFRAAPIRSVDVYNVMCNVVGITPLPNNGSWSRVMCMLKGRASTAPPVWPSHCALALILLFLLA Predict reactive species |
| Full Name | ectonucleotide pyrophosphatase/phosphodiesterase 6 |
| Calculated Molecular Weight | 440 aa, 50 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC035035 |
| Gene Symbol | ENPP6 |
| Gene ID (NCBI) | 133121 |
| RRID | AB_2099784 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UWR7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ENPP6 antibody 12643-1-AP | Download protocol |
| WB protocol for ENPP6 antibody 12643-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ENPP6 Polyclonal antibody (Cat# 12643-1-AP) has 1 total citation. It appears in Experimental & Molecular Medicine (2025), in a study on pulp progenitor cell contributions to odontoblast differentiation and dentin regeneration in murine molars. The research field is dental biology and tissue regeneration, with the antibody applied in immunofluorescence (IF) using mouse samples.
| Species | Application | Title |
|---|---|---|
Exp Mol Med Mx1-labeled pulp progenitor cells are the main contributors of odontoblast and dentin regeneration in murine molars. |
