KD/KO Validated

IkB Beta Polyclonal antibody

IkB Beta Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12660-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

IκB beta, IκB β, NFKBIB, I kappa B beta, IkappaBbeta

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis using 12660-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12660-1-AP (IkB beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 12660-1-AP
WB result of IkB Beta antibody (12660-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-IkB Beta transfected MCF-7 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 12660-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 12660-1-AP (IkB beta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 12660-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 12660-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 12660-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 12660-1-AP (IkB beta antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human endometrial cancer using 12660-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12660-1-AP (IkB beta antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human endometrial cancer using 12660-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12660-1-AP (IkB beta antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 12660-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 12660-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 12660-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IkB Beta antibody (12660-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for IkB Beta in ?

Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells
Positive IHC detected in	human colon tissue, human endometrial cancer tissue, human colon cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12660-1-AP targets IkB Beta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3340 Product name: Recombinant human NFKBIB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-356 aa of BC015528 Sequence: MAGVACLGKAADADEWCDSGLGSLGPDAAAPGGPGLGAELGPGLSWAPLVFGYVTEDGDTALHLAVIHQHEPFLDFLLGFSAGTEYMDLQNDLGQTALHLAAILGETSTVEKLYAAGAGLCVAERRGHTALHLACRVGAHACARALLQPRPRRPREAPDTYLAQGPDRTPDTNHTPVALYPDSDLEKEEEESEEDWKLQLEAENYEGHTPLHVAVIHKDVEMVRLLRDAGADLDKPEPTCGRSPLHLAVEAQAADVLELLLRAGANPAARMYGGRTPLGSAMLRPNPILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRSQTRLPPTPASKPLPDDPRPV Predict reactive species
Full Name	nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
Calculated Molecular Weight	356 aa, 38 kDa
Observed Molecular Weight	46 kDa
GenBank Accession Number	BC015528
Gene Symbol	IkB Beta
Gene ID (NCBI)	4793
RRID	AB_2151611
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15653
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for IkB Beta antibody 12660-1-AP	Download protocol
IHC protocol for IkB Beta antibody 12660-1-AP	Download protocol
WB protocol for IkB Beta antibody 12660-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The IkB Beta polyclonal antibody (Cat# 12660-1-AP) has 2 total citations. These appear in Gut Microbes (2024), studying gut microbiota regulation of host melatonin production via MyD88 in mouse, and Toxicology and Applied Pharmacology (2020), examining salinomycin's promotion of T-cell proliferation by inhibiting indoleamine-2,3-dioxygenase in human breast cancer cells. Both citations used the antibody for Western blot. The associated research fields include microbiome-host interaction, melatonin biology, immunosuppression, and cancer immunology.

Species	Application	Title
mouse	WB	Gut Microbes Gut microbiota regulates host melatonin production through epithelial cell MyD88 Authors - Bingnan Liu View Article
human	WB	Toxicol Appl Pharmacol Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells. Authors - Azubuike Peter Ebokaiwe View Article