Tested Applications
| Positive WB detected in | human brain tissue, human kidney tissue |
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12706-1-AP targets HEBP2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3387 Product name: Recombinant human HEBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-205 aa of BC008205 Sequence: MAEPLQPDPGAAEDAAAQAVETPGWKAPEDAGPQPGSYEIRHYGPAKWVSTSVESMDWDSAIQTGFTKLNSYIQGKNEKEMKIKMTAPVTSYVEPGSGPFSESTITISLYIPSEQQFDPPRPLESDVFIEDRAEMTVFVRSFDGFSSAQKNQEQLLTLASILREDGKVFDEKVYYTAGYNSPVKLLNRNNEVWLIQKNEPTKENE Predict reactive species |
| Full Name | heme binding protein 2 |
| Calculated Molecular Weight | 23 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | BC008205 |
| Gene Symbol | HEBP2 |
| Gene ID (NCBI) | 23593 |
| RRID | AB_2232981 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5Z4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HEBP2(Heme-binding protein 2), also named SOUL and C6orf34, can promote mitochondrial permeability transition and facilitate necrotic cell death under different types of stress conditions (PMID: 17098234). It is a 23 kDa haem-binding protein that was first identified as the PP23 protein isolated from human full-term placentas. hEBP2 is essential for the proliferation of human cells and the repression of hEBP2 severely decreases the ability of EBNA1 and EBV-based plasmids to bind mitotic chromosomes (PMID: 15923612).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for HEBP2 antibody 12706-1-AP | Download protocol |
| WB protocol for HEBP2 antibody 12706-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HEBP2 Polyclonal antibody (Cat# 12706-1-AP) has 1 citation. It was cited in Translational Oncology (IF 4.9, 2024) in a study on high-dimensional single-cell deconstruction of ovarian cancer. The research field encompasses ovarian cancer biology, tumor immune microenvironment (TIME) remodeling, and carboplatin resistance mechanisms. The antibody was applied in Western Blot (WB) for human samples.
| Species | Application | Title |
|---|---|---|
Transl Oncol High-dimensional deconstruction of ovarian cancer at single-cell precision reveals HEBP2 that reshape the TIME and drive carboplatin resistance |
