Tested Applications
| Positive WB detected in | mouse cerebellum tissue, mouse heart tissue, mouse brain tissue, mouse kidney tissue, rat heart tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12714-1-AP targets ZNF238/ZBTB18 in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3406 Product name: Recombinant human ZNF238 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 233-531 aa of BC036677 Sequence: QRSVTSVRDSADVDCVLDLSVKSSLSGVENLNSSYFSSQDVLRSNLVQVKVEKEASCDESDVGTNDYDMEHSTVKESVSTNNRVQYEPAHLAPLREDSVLRELDREDKASDDEMMTPESERVQVEGGMESSLLPYVSNILSPAGQIFMCPLCNKVFPSPHILQIHLSTHFREQDGIRSKPAADVNVPTCSLCGKTFSCMYTLKRHERTHSGEKPYTCTQCGKSFQYSHNLSRHAVVHTREKPHACKWCERRFTQSGDLYRHIRKFHCELVNSLSVKSEALSLPTVRDWTLEDSSQELWK Predict reactive species |
| Full Name | zinc finger protein 238 |
| Calculated Molecular Weight | 531 aa, 59 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC036677 |
| Gene Symbol | ZNF238/ZBTB18 |
| Gene ID (NCBI) | 10472 |
| RRID | AB_2218388 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99592 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZNF238 is a member of the BTB/POZ-ZF protein family, which involve in development and cancer formation, for example BCL-6, PLZF, and HIC-1. It's a transcriptional repressor involve in myogenesis and brain development. By directly repressing the expression of two skeletal myogenesis inhibitors, ID2 and ID3, ZNF238 plays a key role in myogenesis. It can control cell division of progenitor cells and regulating the survival of postmitotic cortical neurons. Besides, ZNF238 involves in the organization of nuclear chromosomes, for its specific binding to the consensus DNA sequence that contains the E box core, and recruiting chromatin remodeling multi-protein complex. ZNF238 proteins has apparent molecular masses of 60 and 48 kD. Specific binding is found for a 60-kDa band which corresponds to the full length of RP58 protein. In addition, a 48-kDa band, thought to be the truncated form 2 is detected (PMID: 9756912).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZNF238/ZBTB18 antibody 12714-1-AP | Download protocol |
| IP protocol for ZNF238/ZBTB18 antibody 12714-1-AP | Download protocol |
| WB protocol for ZNF238/ZBTB18 antibody 12714-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZNF238/ZBTB18 Polyclonal antibody (Cat# 12714-1-AP) has 17 citations. Journals include Nature, Neuron, Molecular Cell, Signal Transduction and Targeted Therapy, Science Advances, Cell Systems, Protein & Cell, Communications Biology, iScience, Life Science Alliance, Cerebral Cortex, Human Mutation, Journal of Pineal Research, Neural Development, MedComm, and PLoS Biology. Research fields span hepatic steatohepatitis, neocortical development, hematopoietic stem cell self-renewal, glioblastoma metabolism, circadian rhythm, neuronal migration, and neuropathic pain.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Hepatic Zbtb18 (Zinc Finger and BTB Domain Containing 18) alleviates hepatic steatohepatitis via FXR (Farnesoid X Receptor)
| ||
Nature Adaptive evolution of gene regulatory networks in mammalian neocortex.
| ||
Protein Cell p21/Zbtb18 repress the expression of cKit to regulate the self-renewal of hematopoietic stem cells
| ||
MedComm (2020) LINC02774 inhibits glycolysis in glioma to destabilize HIF-1α dependent on transcription factor RP58 |
