Tested Applications
| Positive WB detected in | HepG2 cells, human brain tissue, mouse testis tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12719-2-AP targets SKIV2L2 in WB, IHC, IF-P, IP, ELISA, CUT&Tag applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3414 Product name: Recombinant human SKIV2L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3-307 aa of BC028604 Sequence: DAFGDELFSVFEGDSTTAAGTKKDKEKDKGKWKGPPGSADKAGKRFDGKLQSESTNNGKNKRDVDFEGTDEPIFGKKPRIEESITEDLSLADLMPRVKVQSVETVEGCTHEVALPAEEDYLPLKPRVGKAAKEYPFILDAFQREAIQCVDNNQSVLVSAHTSAGKTVCAEYAIALALREKQRVIFTSPIKALSNQKYREMYEEFQDVGLMTGDVTINPTASCLVMTTEILRSMLYRGSEVMREVAWVIFDEIHYMRDSERGVVWEETIILLPDNVHYVFLSATIPNARQFAEWICHLHKQPCHVI Predict reactive species |
| Full Name | superkiller viralicidic activity 2-like 2 (S. cerevisiae) |
| Calculated Molecular Weight | 1042 aa, 118 kDa |
| Observed Molecular Weight | 118 kDa |
| GenBank Accession Number | BC028604 |
| Gene Symbol | SKIV2L2 |
| Gene ID (NCBI) | 23517 |
| RRID | AB_2187479 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42285 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SKIV2L2 antibody 12719-2-AP | Download protocol |
| IHC protocol for SKIV2L2 antibody 12719-2-AP | Download protocol |
| IP protocol for SKIV2L2 antibody 12719-2-AP | Download protocol |
| WB protocol for SKIV2L2 antibody 12719-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SKIV2L2 Polyclonal antibody (Cat# 12719-2-AP) has 9 citations across high-impact journals including Nature, Nature Communications, Nucleic Acids Research, Cell Death & Differentiation, EMBO Journal, Cell Genomics, RNA, Molecular Cell, and iScience. Research fields span rRNA processing, epigenetic regulation, HIV repression, cancer progression, RNA splicing, homologous recombination repair, and vascular biology. Applications cited include WB, IP, IHC, IF, and CUT&Tag, predominantly in human samples.
| Species | Application | Title |
|---|---|---|
Nat Commun TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. | ||
Nucleic Acids Res The NEXT complex regulates H3K27me3 levels to affect cancer progression by degrading G4/U-rich lncRNAs | ||
Cell Death Differ hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis
| ||
EMBO J ALYREF condensation stabilizes m(5)C-modified PARP10 mRNA and promotes PI3K-AKT signaling in ovarian cancer. | ||
Cell Genom NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors |
Reviews
What customers say
Both reviewers gave 5-star ratings. The antibody performed well in Western blot at a 1:1000 dilution on nuclear cell extracts from Jurkat cells. Another user confirmed strong detection of MTR4/SKIV2L2 from HeLa and Jurkat cell lysates by WB (1:1000, 1-hour incubation at room temperature) and successful immunoprecipitation using 4 µg of antibody. Overall, users report reliable performance for both WB and IP applications with no issues noted.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Fabienne (Verified Customer) (05-09-2025) | Works very well in western blot (1:1000 dilution) (tested on nuclear cell extracts)
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Roy (Verified Customer) (06-12-2024) | Great antibody to detect MTR4/SKIV2L2 from HeLa/Jurkat cell extracts by WB (1/1000 - 1h incubation at RT) and did work well to immunoprecipitate MTR4/SKIV2L2 (4µg/IP).
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