Tested Applications
| Positive WB detected in | human placenta tissue, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12752-1-AP targets DPP8 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3262 Product name: Recombinant human DPP8 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-329 aa of BC030688 Sequence: MWKRSEQMKIKSGKCNMAAAMETEQLGVEIFETADCEENIESQDRPKLEPFYVERYSWSQLKKLLADTRKYHGYMMAKAPHDFMFVKRNDPDGPHSDRIYYLAMSGENRENTLFYSEIPKTINRAAVLMLSWKPLLDLFQATLDYGMYSREEELLRERKRIGTVGIASYDYHQGSGTFLFQAGSGIYHVKDGGPQGFTQQPLRPNLVETSCPNIRMDPKLCPADPDWIAFIHSNDIWISNIVTREERRLTYVHNELANMEEDARSAGVATFVLQEEFDRYSGYWWCPKAETTPSGGKILRILYEENDESEVEIIHVTSPMLETRRADSF Predict reactive species |
| Full Name | dipeptidyl-peptidase 8 |
| Calculated Molecular Weight | 847 aa, 97 kDa |
| Observed Molecular Weight | 75-83 kDa |
| GenBank Accession Number | BC030688 |
| Gene Symbol | DPP8 |
| Gene ID (NCBI) | 54878 |
| RRID | AB_10638438 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6V1X1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DPP8 antibody 12752-1-AP | Download protocol |
| IP protocol for DPP8 antibody 12752-1-AP | Download protocol |
| WB protocol for DPP8 antibody 12752-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DPP8 Polyclonal antibody (Cat# 12752-1-AP) has 1 total citation. It was cited in PLoS Pathogens in a 2025 study investigating how the non-structural protein of SFTSV activates the NLRP1 and CARD8 inflammasome by disrupting the DPP9-mediated ternary complex. The research falls within the fields of virology, inflammasome biology, and innate immune signaling. The antibody was used for Western blot analysis in human samples.
| Species | Application | Title |
|---|---|---|
PLoS Pathog The non-structural protein of SFTSV activates NLRP1 and CARD8 inflammasome through disrupting the DPP9-mediated ternary complex. |
