Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human gliomas tissue, human placenta tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12872-1-AP targets ALG1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3540 Product name: Recombinant human ALG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 157-464 aa of BC031095 Sequence: HNYGYSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIRAVTVYDKPASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGSGLVTRLRERPALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPMREYYSRLIHQKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRESQQLRWDESWVRTVLPLVMDT Predict reactive species |
| Full Name | asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae) |
| Calculated Molecular Weight | 464 aa, 53 kDa |
| Observed Molecular Weight | 47-53 kDa |
| GenBank Accession Number | BC031095 |
| Gene Symbol | ALG1 |
| Gene ID (NCBI) | 56052 |
| RRID | AB_2289541 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BT22 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ALG1 (asparagine-linked glycosylation 1) is a β-1,4-mannosyltransferase that catalyzes the committed step of N-linked glycosylation. Operating on the cytoplasmic face of the endoplasmic reticulum, ALG1 transfers the first mannose from GDP-mannose onto dolichol-pyrophosphate-GlcNAc₂, yielding Man₁GlcNAc₂-PP-dolichol. ALG1 is a 464-amino-acid protein with an N-terminal transmembrane domain that is essential for catalytic activity; deleting this domain abolishes mannosyltransferase function.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ALG1 antibody 12872-1-AP | Download protocol |
| IHC protocol for ALG1 antibody 12872-1-AP | Download protocol |
| IP protocol for ALG1 antibody 12872-1-AP | Download protocol |
| WB protocol for ALG1 antibody 12872-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ALG1 Polyclonal antibody (Cat# 12872-1-AP) has 6 citations. These appear in J Agric Food Chem, Int J Mol Sci, Glycoconj J, PLoS One, Oncol Lett, and Am J Med Genet A. Research fields span N-linked glycosylation biology, congenital disorders of glycosylation, lung adenocarcinoma, meibomian gland carcinoma, Friedreich's ataxia, and zinc absorption in food science. All cited applications are WB, with IF and CoIP also represented.
| Species | Application | Title |
|---|---|---|
J Agric Food Chem Structural Characterization of Zinc-Sucrose Complex and Its Ability to Promote Zinc Absorption in Caco-2 Monolayer Cells and Mice | ||
Int J Mol Sci Targeted Proteomics Reveals Quantitative Differences in Low-Abundance Glycosyltransferases of Patients with Congenital Disorders of Glycosylation | ||
Glycoconj J Asparagine-linked glycosylation protein 1 (ALG1) promotes aggressive phenotypes of lung adenocarcinoma cells, A549, via modulating N-linked glycosylation and ER-Stress.
| ||
PLoS One O-GlcNAc-specific antibody CTD110.6 cross-reacts with N-GlcNAc2-modified proteins induced under glucose deprivation.
| ||
Oncol Lett Proteomics reveals the regulation of ALG1 expression by lentivirus-mediated THBS1 overexpression and its mechanism in meibomian gland carcinoma.
| ||
Am J Med Genet A Case Report of Friedreich's Ataxia and ALG1-Related Biochemical Abnormalities in a Patient With Progressive Spastic Paraplegia |
