Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12897-1-AP targets GALNT4 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3590 Product name: Recombinant human GALNT4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 230-578 aa of BC036390 Sequence: CECNSGWLEPLLERIGRDETAVVCPVIDTIDWNTFEFYMQIGEPMIGGFDWRLTFQWHSVPKQERDRRISRIDPIRSPTMAGGLFAVSKKYFQYLGTYDTGMEVWGGENLELSFRVWQCGGKLEIHPCSHVGHVFPKRAPYARPNFLQNTARAAEVWMDEYKEHFYNRNPPARKEAYGDISERKLLRERLRCKSFDWYLKNVFPNLHVPEDRPGWHGAIRSRGISSECLDYNSPDNNPTGANLSLFGCHGQGGNQFFEYTSNKEIRFNSVTELCAEVPEQKNYVGMQNCPKDGFPVPANIIWHFKEDGTIFHPHSGLCLSAYRTPEGRPDVQMRTCDALDKNQIWSFEK Predict reactive species |
| Full Name | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (GalNAc-T4) |
| Calculated Molecular Weight | 578 aa, 67 kDa |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | BC036390 |
| Gene Symbol | GALNT4 |
| Gene ID (NCBI) | 8693 |
| RRID | AB_10643389 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N4A0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GALNT4 is a member of N-acetylgalactosaminyltransferases (GALNTs) which are responsible for initiating step of mucin-type O-glycosylation. Desregulation of GALNTs expression can exert pivotal functions during malignant transformation. It has been reported that GALNT4 expression is markedly repressed in tumoral tissues and lower expression of GALNT4 is significant associated with the adverse survival, indicating its potential role as prognostic factor for cancers like hepatocellular carcinoma.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GALNT4 antibody 12897-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GALNT4 Polyclonal antibody (Cat# 12897-1-AP) has 7 citations. They appear in journals including EMBO Journal, Biochimica et Biophysica Acta (Molecular Basis of Disease), Journal of Molecular and Cellular Cardiology, Cell Signalling, FASEB Journal, FEBS Journal, and Journal of Biological Chemistry. Research fields span O-glycosylation, atherosclerosis, cardiovascular disease, and cancer (non-small cell lung, colorectal, and hepatocellular carcinoma).
| Species | Application | Title |
|---|---|---|
EMBO J The disulfide catalyst QSOX1 maintains the colon mucosal barrier by regulating Golgi glycosyltransferases | ||
Biochim Biophys Acta Mol Basis Dis GALNT4 controls aortic dissection by regulating vascular smooth muscle cell phenotype switch and dysfunction through the TGF-β/smad signaling | ||
J Mol Cell Cardiol GALNT4 primes monocytes adhesion and transmigration by regulating O-Glycosylation of PSGL-1 in atherosclerosis.
| ||
Cell Signal GALNT4 promotes the stemness of non-small cell lung cancer through O-glycosylation of MUC5AC via ITGB4/PI3K/AKT signaling pathway
| ||
FASEB J Galnt4 Knockdown Modulates Lysosomal Function to Reduce Foam Cell Formation and Attenuate Atherosclerotic Plaque Progression | ||
FEBS J The polypeptide N-acetylgalactosaminyltransferase 4 exhibits stage-dependent expression in colorectal cancer and affects tumorigenesis, invasion and differentiation. |
Reviews
What customers say
The product has received one review, rated 5 out of 5 stars. The reviewer used it for immunohistochemistry on human cancer tissue and reported that it works well for IHC. Overall, the feedback is positive, with the antibody performing effectively in its tested application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jennifer (Verified Customer) (04-24-2026) | Works well for IHC
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