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KD/KO Validated

SLC27A3 Polyclonal antibody

SLC27A3 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12943-1-AP

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

ACSVL3, FATP 3, FATP3, Fatty acid transport protein 3, SLC27A3, UNQ367/PRO703, VLCS 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis using 12943-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12943-1-AP (SLC27A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human testis using 12943-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 12943-1-AP (SLC27A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse testis using 12943-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12943-1-AP (SLC27A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse testis using 12943-1-AP
IP result of anti-SLC27A3 (IP:12943-1-AP, 4ug; Detection:12943-1-AP 1:500) with mouse testis tissue lysate 4000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skin cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skin cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skin cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skin cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skin using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse skin using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for SLC27A3 in ?

Overview

Tested Applications
Product Information
Publications (10)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	human testis tissue, mouse testis tissue, MCF-7 cells
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human skin cancer tissue, human breast cancer tissue, mouse skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12943-1-AP targets SLC27A3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4015 Product name: Recombinant human SLC27A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 500-700 aa of BC029792 Sequence: GEPGLLVAPVSQQSPFLGYAGGPELAQGKLLKDVFRPGDVFFNTGDLLVCDDQGFLRFHDRTGDTFRWKGENVATTEVAEVFEALDFLQEVNVYGVTVPGHEGRAGMAALVLRPPHALDLMQLYTHVSENLPPYARPRFLRLQESLATTETFKQQKVRMANEGFDPSTLSDPLYVLDQAVGAYLPLTTARYSALLAGNLRI Predict reactive species
Full Name	solute carrier family 27 (fatty acid transporter), member 3
Calculated Molecular Weight	75 kDa
Observed Molecular Weight	70-79 kDa
GenBank Accession Number	BC029792
Gene Symbol	SLC27A3
Gene ID (NCBI)	11000
RRID	AB_2286200
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5K4L6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC27A3, also named as FATP3, is a member of the solute carrier 27A (SLC27A) gene family which encodes fatty acid transport proteins. SLC27A3 is expressed in human neural stem cells and plays a key role in the developmental stage of the central nervous system. It is associated with autism spectrum disorders (ASD) that caused by impaired early brain development.(PMID: 26548558).

Protocols

Product Specific Protocols
IHC protocol for SLC27A3 antibody 12943-1-AP	Download protocol
IP protocol for SLC27A3 antibody 12943-1-AP	Download protocol
WB protocol for SLC27A3 antibody 12943-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Anti-SLC27A3 antibody (Cat# 12943-1-AP) has 10 total citations spanning journals including Nature Medicine, J Neuroinflammation, Frontiers in Immunology, European Journal of Pharmacology, International Journal of Molecular Sciences, Cancers, Molecular Reproduction and Development, Biochemical and Biophysical Research Communications, bioRxiv, and Advanced Science. Research fields cover lipid metabolism, insulin resistance, neuroinflammation, COPD, hepatic cholestasis, adipose browning, breast cancer, reproductive biology, pancreatic cancer, KRAS-targeted therapy, and glioblastoma.

Species	Application	Title
human	WB	Nat Med PMID: 26950361 Authors - Cholsoon Jang KD Validated View Article
		J Neuroinflammation SLC27A3-dependent lipid metabolic reprogramming by trilobatin suppresses microglial mtDNA/TLR9-driven inflammatory activation in traumatic brain injury. Authors - Hui-Wen Zhang View Article
human,mouse	WB,IHC	Front Immunol Machine-Learning Algorithm-Based Prediction of Diagnostic Gene Biomarkers Related to Immune Infiltration in Patients With Chronic Obstructive Pulmonary Disease. Authors - Yuepeng Zhang View Article
mouse	WB	Eur J Pharmacol A novel therapy for hepatic cholestasis treatment-the combination of rosiglitazone and fenofibrate Authors - Yuanli Chen View Article
mouse	WB	Int J Mol Sci Knockdown of VEGFB/VEGFR1 Signaling Promotes White Adipose Tissue Browning and Skeletal Muscle Development Authors - Mingfa Ling View Article
mouse	WB	Cancers (Basel) In Vivo Optical Metabolic Imaging of Long-Chain Fatty Acid Uptake in Orthotopic Models of Triple-Negative Breast Cancer. Authors - Megan C Madonna View Article