Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12954-1-AP targets FANCM in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3618 Product name: Recombinant human FANCM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC036056 Sequence: MSGRQRTLFQTWGSSISRSSGTPGCSSGTERPQSPGSSKAPLPAAAEAQLESDDDVLLVAAYEAERQLCLENGGFCTSAGALWIYPTNCPVRDYQLHISRAALFCNTLVCLPTGLGKTFIAAVVMYNFYRWFPSGKVVFMAPTKPLVTQQIEACYQVMGIPQSHMAEMTGSTQASTRKEIWCSKRVLFLTPQVMVNDLSRGACPAAEIKCLVIDEAHKALGNYAYCQVVRELVKYTNHFRILALSATPGSDIKAVQQVITNLLIGQIELRSEDSPDILTYSHERKVEKLIVPLGEELAAI Predict reactive species |
| Full Name | Fanconi anemia, complementation group M |
| Calculated Molecular Weight | 2048 aa, 232 kDa |
| Observed Molecular Weight | 250 kDa, 232 kDa |
| GenBank Accession Number | BC036056 |
| Gene Symbol | FANCM |
| Gene ID (NCBI) | 57697 |
| RRID | AB_10644146 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IYD8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FANCM (Fanconi anemia group M protein) is a DNA-dependent ATPase component of the Fanconi anemia (FA) core complex (PMID: 16116422). It is required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage (PMID:16116422; 19423727; 20347428). FANCM is localized in the cell nucleus and can bind double-stranded DNA (dsDNA), fork-structured DNA (fsDNA), and Holliday junction substrates (PMID: 20347428; 20347429).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FANCM antibody 12954-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FANCM Polyclonal antibody (Cat# 12954-1-AP) has 5 total citations, published in EMBO Journal, Cell Proliferation, EMBO Reports, mSystems, and OncoTargets and Therapy. The cited research covers RNA m6A modification and regulation, stress granule biology, host–pathogen interaction, and gastric cancer oncology.
| Species | Application | Title |
|---|---|---|
EMBO J m6 A deposition is regulated by PRMT1-mediated arginine methylation of METTL14 in its disordered C-terminal region. | ||
Cell Prolif Lin28a forms an RNA-binding complex with Igf2bp3 to regulate m6A-modified stress response genes in stress granules of muscle stem cells | ||
EMBO Rep Arginine methylation-dependent METTL14-SMN interaction regulates RNA m(6)A homeostasis. | ||
mSystems Dual RNA sequencing of Helicobacter pylori and host cell transcriptomes reveals ontologically distinct host-pathogen interaction | ||
Onco Targets Ther The Regulatory Mechanism and Biological Significance of Mitochondrial Calcium Uniporter in the Migration, Invasion, Angiogenesis and Growth of Gastric Cancer. |
