Tested Applications
| Positive WB detected in | mouse testis tissue, HEK-293 cells, HeLa cells, human spleen tissue, mouse heart tissue, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12984-1-AP targets PHD1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3616 Product name: Recombinant human EGLN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 190-407 aa of BC036051 Sequence: GICVKDSFLGAALGGRVLAEVEALKRGGRLRDGQLVSQRAIPPRSIRGDQIAWVEGHEPGCRSIGALMAHVDAVIRHCAGRLGSYVINGRTKAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVANIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAAKDKYQLASGQKGVQVPVSQPPTPT Predict reactive species |
| Full Name | egl nine homolog 2 (C. elegans) |
| Calculated Molecular Weight | 407 aa, 44 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC036051 |
| Gene Symbol | PHD1 |
| Gene ID (NCBI) | 112398 |
| RRID | AB_2096849 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96KS0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EGLN2, also named as PHD1, EIT6 and HPH-3, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN2 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.
Publications
What published studies show
The PHD1 (EGLN2) polyclonal antibody (Cat# 12984-1-AP) has 13 citations published across 13 journals, including Cancer Cell, Cell Research, Cell Death & Disease, Cell Communications & Signaling, Journal of Translational Medicine, Cells, Frontiers in Endocrinology, Respiratory Research, American Journal of Pathology, Journal of Cellular and Molecular Medicine, Journal of Gene Medicine, Asian Journal of Andrology, and Leukemia & Lymphoma. Research fields span cancer biology, hypoxia/HIF signaling, fibrosis, metabolism, inflammation, and renal injury.
| Species | Application | Title |
|---|---|---|
Cancer Cell Targeting P4HA1 promotes CD8+ T cell progenitor expansion toward immune memory and systemic anti-tumor immunity | ||
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation | ||
Cell Death Dis Cancer associated fibroblast-derived CCL5 promotes hepatocellular carcinoma metastasis through activating HIF1α/ZEB1 axis. | ||
Cell Commun Signal Oncometabolite 2-hydroxyglutarate destabilizes CDH1 to regulate quiescence and enhance chemotherapy sensitivity.
| ||
J Transl Med Cancer-associated fibroblast-secreted FGF7 as an ovarian cancer progression promoter | ||
Cells Pseudohypoxia-Stabilized HIF2α Transcriptionally Inhibits MNRR1, a Druggable Target in MELAS |
Reviews
What customers say
The product received a single 5-star review for Western Blot applications. The reviewer used a 1:500 dilution on SH-SY5Y cells and reported a fully positive result, indicating strong performance and reliability for WB use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vignesh (Verified Customer) (11-18-2025) |
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