Tested Applications
| Positive WB detected in | PC-3 cells, HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3200 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13044-1-AP targets GNS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3712 Product name: Recombinant human GNS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 200-552 aa of BC012482 Sequence: SVDYLTDVLANVSLDFLDYKSNFEPFFMMIATPAPHSPWTAAPQYQKAFQNVFAPRNKNFNIHGTNKHWLIRQAKTPMTNSSIQFLDNAFRKRWQTLLSVDDLVEKLVKRLEFTGELNNTYIFYTSDNGYHTGQFSLPIDKRQLYEFDIKVPLLVRGPGIKPNQTSKMLVANIDLGPTILDIAGYDLNKTQMDGMSLLPILRGASNLTWRSDVLVEYQGEGRNVTDPTCPSLSPGVSQCFPDCVCEDAYNNTYACVRTMSALWNLQYCEFDDQEVFVEVYNLTADPDQITNIAKTIDPELLGKMNYRLMMLQSCSGPTCRTPGVFDPGYRFDPRLMFSNRGSVRTRRFSKHLL Predict reactive species |
| Full Name | glucosamine (N-acetyl)-6-sulfatase |
| Calculated Molecular Weight | 552 aa, 62 kDa |
| Observed Molecular Weight | 94 kDa |
| GenBank Accession Number | BC012482 |
| Gene Symbol | GNS |
| Gene ID (NCBI) | 2799 |
| RRID | AB_2115308 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15586 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GNS antibody 13044-1-AP | Download protocol |
| IHC protocol for GNS antibody 13044-1-AP | Download protocol |
| WB protocol for GNS antibody 13044-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GNS Polyclonal antibody (Cat# 13044-1-AP) has 1 citation. It was cited in Nature Communications in a study investigating how lysosomal protease deficiency or substrate overload induces an oxidative-stress-mediated STAT3-dependent pathway of lysosomal homeostasis. The research field encompasses lysosomal biology, oxidative stress signaling, and cell homeostasis. The antibody was applied in Western Blot using mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Lysosomal protease deficiency or substrate overload induces an oxidative-stress mediated STAT3-dependent pathway of lysosomal homeostasis. |
