Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13047-1-AP targets DDX11 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3666 Product name: Recombinant human DDX11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-345 aa of BC011264 Sequence: MANETQKVGAIHFPFPFTPYSIQEDFMAELYRVLEAGKIGIFESPTGTGKSLSLICGALSWLRDFEQKKREEEARLLETGTGPLHDEKDESLCLSSSCEGAAGTPRPAGEPAWVTQFVQKKEERDLVDRLKAEQARRKQREERLQQLQHRVQLKYAAKRLRQEEEERENLLRLSREMLETGPEAERLEQLESGEEELVLAEYESDEEKKVASRVDEDEDDLEEEHITKIYYCSRTHSQLAQFVHEVKKSPFGKDVRLVSLGSRQNLCVNEDVKSLGSVQLINDRCVDMQRSRHEKKKGAEEEKPKRRRQEKQAACPFYNHEQMGLLRDEALAEVKDMEQLLALGK Predict reactive species |
| Full Name | DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae) |
| Calculated Molecular Weight | 970 aa, 108 kDa |
| Observed Molecular Weight | 102-108 kDa |
| GenBank Accession Number | BC011264 |
| Gene Symbol | DDX11 |
| Gene ID (NCBI) | 1663 |
| RRID | AB_3741838 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96FC9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DDX11 (DEAD/H-box helicase 11), also known as hCHLR1, KRG-2, is a member of the DExD/H (Asp-Glu-x-Asp/His)-box helicase family containing DNA helicase with roles in DNA replication and sister chromatid cohesion establishment, and general chromosome architecture (PMID: 33802088). DDX11 encodes an iron-sulfur cluster DNA helicase required for development, mutated, and overexpressed in cancers (PMID: 33879618). DDX11 is also a positive regulator of the RIG-I-mitochondrial antiviral signaling protein (MAVS)-mediated signaling pathways (PMID: 39470258).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX11 antibody 13047-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DDX11 Polyclonal antibody (Cat# 13047-1-AP) has 1 total citation. It was cited in Cytojournal (2025) in a study investigating how the DDX11-ATAD5 interaction mediates epithelial-mesenchymal transition to promote gallbladder cancer progression. The associated research field is cancer biology, specifically tumor metastasis and EMT in gallbladder carcinoma. The antibody was used for Western blot in human samples with RNAi validation.
| Species | Application | Title |
|---|---|---|
Cytojournal Novel mechanism of tumor metastasis: DDX11-ATAD5 interaction mediates epithelial-mesenchymal transition to promote gallbladder cancer progression.
|
