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Cadherin-18 Polyclonal antibody (13091-1-AP)

Cadherin-18 Polyclonal antibody

Cadherin-18 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13091-1-AP

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

CDH18, cadherin 18, type 2, Cadherin-14, CDH14, CDH14L

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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WB analysis using 13091-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13091-1-AP (Cadherin-18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human gliomas using 13091-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13091-1-AP (Cadherin-18 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human gliomas using 13091-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13091-1-AP (Cadherin-18 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat cerebellum using 13091-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 13091-1-AP (Cadherin-18 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat cerebellum using 13091-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 13091-1-AP (Cadherin-18 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, rat brain tissue
Positive IHC detected in	human gliomas tissue, rat cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13091-1-AP targets Cadherin-18 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3675 Product name: Recombinant human CDH18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-382 aa of BC031051 Sequence: TAHHSSIKVMRNQTKHIEGETEVHHRPKRGWVWNQFFVLEEHMGPDPQYVGKLHSNSDKGDGSVKYILTGEGAGTIFIIDDTTGDIHSTKSLDREQKTHYVLHAQAIDRRTNKPLEPESEFIIKVQDINDNAPKFTDGPYIVTVPEMSDMGTSVLQVTATDADDPTYGNSARVVYSILQGQPYFSVDPKTGVIRTALHNMDGEAREHYSVVIQAKDMAGQVGGLSGSTTVNITLTDVNDNTPRFPQKHYQLYVPESAQVGSAVGKIKANDADTGSNADMTYSIINGDGMGIFSISTDKETREGILSLKKPLNYEKKKSYTLNIEGANTHLDFRFSHLGPFKDATMLKIIVGDVDEPPL Predict reactive species
Full Name	cadherin 18, type 2
Calculated Molecular Weight	790 aa, 88 kDa
Observed Molecular Weight	105 kDa
GenBank Accession Number	BC031051
Gene Symbol	Cadherin-18
Gene ID (NCBI)	1016
RRID	AB_10646435
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13634
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for Cadherin-18 antibody 13091-1-AP	Download protocol
WB protocol for Cadherin-18 antibody 13091-1-AP	Download protocol

Standard Protocols
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Publications

What published studies show

Anti-CDH18 (catalog no. 13091-1-AP) has a total of 3 citations, appearing in NPJ Regen Med, Front Mol Biosci, and Cell Physiol Biochem. The associated research fields encompass vascular smooth muscle cell differentiation, uterine corpus endometrial carcinoma prognosis, and glioma cell invasiveness.

Species	Application	Title
human	WB	NPJ Regen Med CDH18 is a fetal epicardial biomarker regulating differentiation towards vascular smooth muscle cells. Authors - Julia Junghof View Article
human	IF	Front Mol Biosci Calcium-dependent adhesion protein CDH18, a potential biomarker for prognosis in uterine corpus endometrial carcinoma Authors - Xiaoxuan Tang View Article
human	WB,IHC	Cell Physiol Biochem A Novel Tumor-Suppressor, CDH18, Inhibits Glioma Cell Invasiveness Via UQCRC2 and Correlates with the Prognosis of Glioma Patients. Authors - Ya-Hui Bai View Article