Tested Applications
| Positive WB detected in | A431 cells, SGC-7901 cells, human stomach tissue, A549 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HCT 116 cells, A431 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13149-1-AP targets FBXW11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3761 Product name: Recombinant human FBXW11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 216-529 aa of BC026213 Sequence: NLQRIQCRSENSKGVYCLQYDDEKIISGLRDNSIKIWDKTSLECLKVLTGHTGSVLCLQYDERVIVTGSSDSTVRVWDVNTGEVLNTLIHHNEAVLHLRFSNGLMVTCSKDRSIAVWDMASATDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWSTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLQAALDPRAPASTLCLRTLVEHSGRVFRLQFDEFQIISSSHDDTILIWDFLNVPPSAQNETRSPSRTYTYISR Predict reactive species |
| Full Name | F-box and WD repeat domain containing 11 |
| Calculated Molecular Weight | 542 aa, 61 kDa |
| Observed Molecular Weight | 61 kDa |
| GenBank Accession Number | BC026213 |
| Gene Symbol | FBXW11 |
| Gene ID (NCBI) | 23291 |
| RRID | AB_2102595 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKB1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FBXW11 (also known as HOS or β-TrCP2) is a member of F-box family proteins and plays critical role in regulating the ubiquitination of phosphorylated substrates. Abnormal expression of several FBXW11 is involved in the modulation of various biological events, such as cell cycle, differentiation, migration, inflammation, and apoptosis, through targeting multiple different substrates. For instance, FBXW11 could bind to the phosphorylated IκB and β-catenin, promoting their degradation via the ubiquitin-proteasome system. In addition, FBXW11 expression is markedly increased in mouse skin tumors and promotes tumor growth by activating the NF-κB signaling (PMID: 33640602). FBXW11 has 3 isoforms with the molecular mass of 58-62 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FBXW11 antibody 13149-1-AP | Download protocol |
| IHC protocol for FBXW11 antibody 13149-1-AP | Download protocol |
| WB protocol for FBXW11 antibody 13149-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
FBXW11 Polyclonal antibody (Cat# 13149-1-AP) has 20 citations across journals including Nature Communications, Science Advances, Cell Death & Differentiation, EMBO Journal, Signal Transduction and Targeted Therapy, Cell Reports, and Journal of Immunology. Research fields span oncology (lung, ovarian, breast, colorectal, pancreatic, and bladder cancers), protein ubiquitination and degradation, cell cycle regulation, neurogenesis, inflammation, and immune signaling.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Tet methylcytosine dioxygenase 2 (TET2) deficiency elicits EGFR-TKI (tyrosine kinase inhibitors) resistance in non-small cell lung cancer | ||
Nat Commun The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor. | ||
Sci Adv WWC proteins-mediated compensatory mechanism restricts schwannomatosis driven by NF2 loss of function | ||
Cell Death Differ NUMB facilitates autophagy initiation through targeting SCFβ-TrCP2 complex. | ||
J Pharm Anal E3 ubiquitin ligase FBXW11-mediated downregulation of S100A11 promotes sensitivity to PARP inhibitor in ovarian cancer.
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