Tested Applications
| Positive WB detected in | human heart tissue, mouse spleen tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13199-2-AP targets PDLIM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3934 Product name: Recombinant human PDLIM3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC027870 Sequence: MPQTVILPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAAAHQLCLKIDRGETHLWSPQVSEDGKAHPFKINLESEPQEFKPIGTAHNRRAQPFVAAANIDDKRQVVSASYNSPIGLYSTSNIQDALHGQLRGLIPSSPQNEPTASVPPESDVYRMLHDNRNEPTQPRQSGSFRVLQGMVDDGSDDRPAGTRSVRAPVTKVHGGSGGAQRMPLCDKCGSGIVGAVVKARDKYRHPECFVCADCNLNLKQKGYFFIEGELYCETHARARTKPPEGYDTVTLYPKA Predict reactive species |
| Full Name | PDZ and LIM domain 3 |
| Calculated Molecular Weight | 316 aa, 34 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC027870 |
| Gene Symbol | PDLIM3 |
| Gene ID (NCBI) | 27295 |
| RRID | AB_2161653 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q53GG5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDLIM3, also known as ALP, contains a PDZ domain and a LIM domain. PDLIM3 binds the spectrin-like repeats of alpha-actinin-2 and colocalizes with alpha-actinin-2 at the Z lines of skeletal muscle.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDLIM3 antibody 13199-2-AP | Download protocol |
| WB protocol for PDLIM3 antibody 13199-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PDLIM3 antibody (Cat# 13199-2-AP) has 5 total citations across journals including Gut Microbes, Small, J Tissue Eng, Int J Mol Sci, and Cell Mol Biol. Research fields span gut dysbiosis and colitis, osteoporotic bone regeneration via mitophagy, vascularization and tissue engineering, head and neck squamous cell carcinoma (EMT), and osteoblast proliferation/differentiation. Most studies applied the antibody in Western blot with mouse samples.
| Species | Application | Title |
|---|---|---|
Gut Microbes Ultra-processed foods sourced 7-ketositosterol aggravates colitis through gut dysbiosis induced-PDLIM3 activation. | ||
Small Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy | ||
J Tissue Eng Regulation of the immune microenvironment by pioglitazone-loaded polylactic glycolic acid nanosphere composite scaffolds to promote vascularization and bone regeneration | ||
Int J Mol Sci PDLIM3 Regulates Migration and Invasion of Head and Neck Squamous Cell Carcinoma via YAP-Mediated Epithelial-Mesenchymal Transition | ||
Cell Mol Biol (Noisy-le-grand) Isofraxidin inhibits the proliferation and differentiation of the osteoblast MC3T3-E1 subclone 14 cell line |
