Tested Applications
| Positive WB detected in | mouse brain tissue, MCF-7 cells |
| Positive IP detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13235-1-AP targets ATP6V1E2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4088 Product name: Recombinant human ATP6V1E2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-226 aa of BC034808 Sequence: MALSDVDVKKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKILMSTMRNQARLKVLRARNDLISDLLSEAKLRLSRIVEDPEVYQGLLDKLVLQGLLRLLEPVMIVRCRPQDLLLVEAAVQKAIPEYMTISQKHVEVQIDKEAYLAVNAAGGVEVYSGNQRIKVSNTLESRLDLSAKQKMPEIRMALFGANTNRKFFI Predict reactive species |
| Full Name | ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2 |
| Calculated Molecular Weight | 226 aa, 26 kDa |
| Observed Molecular Weight | 31-33 kDa |
| GenBank Accession Number | BC034808 |
| Gene Symbol | ATP6V1E2 |
| Gene ID (NCBI) | 90423 |
| RRID | AB_2062675 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96A05 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ATP6V1E2 antibody 13235-1-AP | Download protocol |
| WB protocol for ATP6V1E2 antibody 13235-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATP6V1E2 Polyclonal antibody (Cat# 13235-1-AP) has 1 citation to date. The cited publication appeared in Scientific Reports (2025) and investigated the involvement of the synaptic vesicle cycle in homocysteine-induced neurotoxicity in vitro and in vivo. The research field is neuroscience, specifically focusing on synaptic function and neurotoxicity mechanisms. The antibody was applied by Western blot in mouse and rat samples.
| Species | Application | Title |
|---|---|---|
Sci Rep The involvement of the synaptic vesicle cycle in homocysteine induced neurotoxicity in vitro and in vivo |
