Tested Applications
| Positive WB detected in | mouse liver tissue, human placenta tissue, rat liver tissue |
| Positive IHC detected in | human stomach cancer tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13243-1-AP targets CYP4F12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3957 Product name: Recombinant human CYP4F12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 225-524 aa of BC035350 Sequence: ILELSALVEKRSQHILQHMDFLYYLSHDGRRFHRACRLVHDFTDAVIRERRRTLPTQGIDDFFKDKAKSKTLDFIDVLLLSKDEDGKALSDEDIRAEADTFMFGGHDTTASGLSWVLYNLARHPEYQERCRQEVQELLKDRDPKEIEWDDLAQLPFLTMCVKESLRLHPPAPFISRCCTQDIVLPDGRVIPKGITCLIDIIGVHHNPTVWPDPEVYDPFRFDPENSKGRSPLAFIPFSAGPRNCIGQAFAMAEMKVVLALMLLHFRFLPDHTEPRRKLELIMRAEGGLWLRVEPLNVSLQ Predict reactive species |
| Full Name | cytochrome P450, family 4, subfamily F, polypeptide 12 |
| Calculated Molecular Weight | 524 aa, 60 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | BC035350 |
| Gene Symbol | CYP4F12 |
| Gene ID (NCBI) | 66002 |
| RRID | AB_2088412 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HCS2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CYP4F12(Cytochrome P450 4F12) Catalyzes leukotriene B4 omega-hydroxylation and arachidonic acid omega-hydroxylation but with an activity much lower than that of CYP4F2. It expresses a band of 60KDa in adult and fetal liver, kidney, placenta at term, seminal vesicles, and the prostate gland(PMID:15078342). And it can be phosphorylated in vivo(PMID:18828626).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CYP4F12 antibody 13243-1-AP | Download protocol |
| IHC protocol for CYP4F12 antibody 13243-1-AP | Download protocol |
| WB protocol for CYP4F12 antibody 13243-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CYP4F12 Polyclonal antibody (Cat# 13243-1-AP) has 2 citations. These appear in Scientific Reports and Cancer Genomics Proteomics. The cited research covers oncology fields, specifically head and neck squamous cell carcinoma involving EMT pathway and cell migration, and hepatocellular carcinoma where ALDH1A1 was identified as an oncogenic driver. Both studies used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Sci Rep CYP4F12 is a potential biomarker and inhibits cell migration of head and neck squamous cell carcinoma via EMT pathway | ||
Cancer Genomics Proteomics Integrated Proteomics Reveal ALDH1A1 as an Oncogenic Driver and Regulatory Hub in Hepatocellular Carcinoma. |
