Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13367-1-AP targets SPA17 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4186 Product name: Recombinant human SPA17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-151 aa of BC032457 Sequence: MSIPFSNTHYRIPQGFGNLLEGLTREILREQPDNIPAFAAAYFESLLEKREKTNFDPAEWGSKVEDRFYNNHAFEEQEPPEKSDPKQEESQISGKEEETSVTILDSSEEDKEKEEVAAVKIQAAFRGHIAREEAKKMKTNSLQNEEKEENK Predict reactive species |
| Full Name | sperm autoantigenic protein 17 |
| Calculated Molecular Weight | 151 aa, 17 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC032457 |
| Gene Symbol | SPA17 |
| Gene ID (NCBI) | 53340 |
| RRID | AB_2194631 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15506 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sperm surface protein Sp17(SPA17)is a 151 amino acids protein (17kDa), which is a sperm surface peripheral membrane protein. It is a highly conserved mammalian protein expressed in developing spermatozoa and mature spermatids, and reported to play a role in the interaction of sperm with the zona pellucida. SP17 exists as a homodimer and localizes to the head and tail of spermatozoa. SP17 has been identified as a cancer/testis antigen and is expressed in ovarian cancer and multiple myeloma. This suggests that SP17 could be suitable as a target in tumor immunotherapy.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SPA17 antibody 13367-1-AP | Download protocol |
| IP protocol for SPA17 antibody 13367-1-AP | Download protocol |
| WB protocol for SPA17 antibody 13367-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SPA17 Polyclonal antibody (Cat# 13367-1-AP) has 7 total citations. These appear in Nature, Phytomedicine, British Journal of Cancer, Frontiers in Immunology, Scientific Reports, BMC Cancer, and Asian Journal of Andrology. Research fields span breast tumour metastasis, male infertility and spermatogenesis, hepatocellular carcinoma, pan-cancer immunotherapy response, ovarian cancer biomarker discovery, and sperm capacitation signaling.
| Species | Application | Title |
|---|---|---|
Phytomedicine Chinese medicine Sheng Jing Decoction alleviates oligoasthenospermia by modulating PI3K/Akt and MAPK signaling pathways. | ||
Br J Cancer Tumour antigen expression in hepatocellular carcinoma in a low-endemic western area. | ||
Front Immunol Sperm Autoantigenic Protein 17 Predicts the Prognosis and the Immunotherapy Response of Cancers: A Pan-Cancer Analysis. | ||
Sci Rep Testis-specific peroxiredoxin 4 variant is not absolutely required for spermatogenesis and fertility in mice. | ||
BMC Cancer Validity and prognostic significance of sperm protein 17 as a tumor biomarker for epithelial ovarian cancer: a retrospective study. |
