SCFD2 Polyclonal antibody

SCFD2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13375-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Sec1 family domain-containing protein 2, STXBP1L1, Syntaxin-binding protein 1-like 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis using 13375-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13375-1-AP (SCFD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of COLO 320 using 13375-1-AP
IP result of anti-SCFD2 (IP:13375-1-AP, 4ug; Detection:13375-1-AP 1:600) with COLO 320 cells lysate 1680 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 13375-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 13375-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 13375-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13375-1-AP (SCFD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 13375-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 13375-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 13375-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	COLO 320 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IP detected in	COLO 320 cells
Positive IHC detected in	human colon cancer tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13375-1-AP targets SCFD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4197 Product name: Recombinant human SCFD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC032453 Sequence: MSASGVLSFTQQGWEQVLAKVKRAVVYLDAACAESLHWGCGSTRLLEAVGGPDCHLREFEPDAIGGGAKQPKAVFVLSCLLKGRTVEILRDIICRSHFQYCVVVTTVSHAVHLTANHVPAAAAAEMEGQQPVFEQLEEKLCEWMGNMNYTAEVFHVPLLLAPVAPHFALTPAFASLFPLLPQDVHLLNSARPDKRKLGSLGDVDSTTLTPELLLQIRCLVSGLSSLCEHLGVREECFAVGSLSQVIAADLANYAPAKNRKKTAAGRASVVFVDRTLDLTGAVGHHGDNLVEKIISALPQLPGHTNDVMVNMIALTALHTEEENYNVVAPGCLSQSSDTTAKALWEALLNTK Predict reactive species
Full Name	sec1 family domain containing 2
Calculated Molecular Weight	684 aa, 75 kDa
Observed Molecular Weight	70-75 kDa
GenBank Accession Number	BC032453
Gene Symbol	SCFD2
Gene ID (NCBI)	152579
RRID	AB_10646473
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WU76
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for SCFD2 antibody 13375-1-AP	Download protocol
IP protocol for SCFD2 antibody 13375-1-AP	Download protocol
WB protocol for SCFD2 antibody 13375-1-AP	Download protocol

Standard Protocols
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Publications

What published studies show

The SCFD2 Polyclonal antibody (Cat# 13375-1-AP) has 1 citation. It was used in a study published in Molecular Biology of the Cell (2026), which employed an integrated proximity labeling and vesicle reconstitution assay to identify novel regulators of Sonic hedgehog secretion. The research field is developmental biology and Hedgehog signaling pathway, with the antibody applied in Western blot for human samples.

Species	Application	Title
human	WB	Mol Biol Cell An integrated proximity labeling and vesicle reconstitution assay identifies novel regulators of Sonic hedgehog secretion. Authors - Ziyang Song KD Validated View Article