Tested Applications
| Positive WB detected in | human liver tissue, human kidney tissue |
| Positive IP detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13380-1-AP targets MUC20 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4205 Product name: Recombinant human MUC20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 255-555 aa of BC029267 Sequence: TLLAEALVTVTNIEVINCSITEIETTTSSIPGASDTDPIPTEGVKASSTSDPPALPDSTEAKPHITEVTASAETLSTAGTTESAAPDATIGTPLPTNSTIEREVTAPGATTLSGALATGNPLEETSALSVETPSYVKVSGAAPVSIEAGSAVGKTTSFAGSSASSYSPSEAALKNFTPSETLTTDIATKGPFPTSRDPLPSVPPTTTNSSRGTNSTLAKITTSAKTTMKPPTATPTTARTRPTTDMSAGENGGFLLLRLSVASPEDLTDPRVAERLMQQLHRELHAHAPHFQVSLLRVRRG Predict reactive species |
| Full Name | mucin 20, cell surface associated |
| Calculated Molecular Weight | 55 kDa, 71 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC029267 |
| Gene Symbol | MUC20 |
| Gene ID (NCBI) | 200958 |
| RRID | AB_2235526 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N307 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MUC20 contains serine, threonine, and proline-rich repeats in its extracellular domain and is likely to be extensively O-glycosylated. MUC20 blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway and inhibiting HGF-induced proliferation of MMP1 and MMP9 expression (PMID: 15314156). MUC20 is highly expressed in the kidney and localized in the proximal tubules but not in the glomerulus or distal tubules(PMID: 14565953). MUC20 was detected in most of the male urogenital tract epithelia, except for epididymis(PMID: 16997931). The expression of MUC20 is up-regulated in the kidneys of patients with immunoglobulin A nephropathy, in an animal model of lupus nephritis, and in mice with acute renal injury caused by cisplatin administration or unilateral ureteral obstruction(PMID: 14565953).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for MUC20 antibody 13380-1-AP | Download protocol |
| WB protocol for MUC20 antibody 13380-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MUC20 Polyclonal antibody (Cat# 13380-1-AP) has 1 citation, published in Journal of Experimental & Clinical Cancer Research (IF 14.3). The study investigated how MUC20, regulated by extrachromosomal circular DNA, attenuates proteasome inhibitor resistance in multiple myeloma by modulating cuproptosis. The antibody was used in Western blot on human samples. The associated research field is cancer biology, specifically multiple myeloma and cell death mechanisms.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res MUC20 regulated by extrachromosomal circular DNA attenuates proteasome inhibitor resistance of multiple myeloma by modulating cuproptosis |
