Tested Applications
| Positive WB detected in | HEK-293 cells, COLO 320 cells, HeLa cells, K-562 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human skin cancer tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13382-1-AP targets NCAPD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4219 Product name: Recombinant human NCAPD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC028182 Sequence: MAPQMYEFHLPLSPEELLKSGGVNQYVVQEVLSIKHLPPQLRAFQAAFRAQGPLAMLQHFDTIYSILHHFRSIDPGLKEDTLEFLIKVVSRHSQELPAILDDTTLSGSDRNAHLNALKMNCYALIRLLESFETMASQTNLVDLDLGGKGKKARTKAAHGFDWEEERQPILQLLTQLLQLDIRHLWNHSIIEEEFVSLVTGCCYRLLENPTINHQKNRPTREAITHLLGVALTRYNHMLSATVKIIQMLQHFEHLAPVLVAAVSLWATDYGMKSIVGEIVREIGQKCPQELSRDPSGTKGFAAFLTELAERVPAILMSSMCILLDHLDGENYMMRNAVLAAMAEMVLQVLSG Predict reactive species |
| Full Name | non-SMC condensin I complex, subunit D2 |
| Calculated Molecular Weight | 1401 aa, 157 kDa |
| Observed Molecular Weight | 157 kDa |
| GenBank Accession Number | BC028182 |
| Gene Symbol | NCAPD2 |
| Gene ID (NCBI) | 9918 |
| RRID | AB_2149840 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15021 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NCAPD2 is a regulatory subunit of the condensin complex, which is required for conversion of interphase chromatin into mitotic-like condense chromosomes. Condensin complexes are heteropentamers comprised of two SMC (structural maintenance of chromosomes) subunits and three non-SMC subunits. The C-terminus of NCAPD2 interacts with Histones H1 and H3 through their histone tails. A loss of NCAPD2 can lead to the disorganization of chromatid axes, misalignment of sister chromatids during metaphase and delayed entry into anaphase.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NCAPD2 antibody 13382-1-AP | Download protocol |
| IHC protocol for NCAPD2 antibody 13382-1-AP | Download protocol |
| IP protocol for NCAPD2 antibody 13382-1-AP | Download protocol |
| WB protocol for NCAPD2 antibody 13382-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-NCAPD2 (catalog #13382-1-AP) has 8 citations published in Cancer Letters, International Dental Journal, Cell Signaling, iScience, BBA Molecular Cell Research, American Journal of Cancer Research, Digestive Diseases and Sciences, and Aging. Research fields span cancer biology (colorectal, oral, esophageal, ovarian, gastric, and liver cancers), autophagy regulation, glycolysis, EMT, Wnt/Notch signaling, bone aging, and tumor immunology.
| Species | Application | Title |
|---|---|---|
Cancer Lett NCAPD2 inhibits autophagy by regulating Ca2+/CAMKK2/AMPK/mTORC1 pathway and PARP-1/SIRT1 axis to promote colorectal cancer.
| ||
Int Dent J Impact of Non-SMC Condensin I Complex Subunit D2 Upregulation on Oral Squamous Cell Carcinoma Prognosis | ||
Cell Signal NCAPD2 stimulates glycolysis and esophageal cancer metastasis through Wnt5A-dependent Notch activation.
| ||
iScience KDM3A and KDM4C Regulate Mesenchymal Stromal Cell Senescence and Bone Aging via Condensin-mediated Heterochromatin Reorganization. | ||
Biochim Biophys Acta Mol Cell Res NCAPD2 facilitates ovarian cancer progression via MYC-mediated unfolded protein response. | ||
Am J Cancer Res High glucose-induced NCAPD2 upregulation promotes malignant phenotypes and regulates EMT via the Wnt/β-catenin signaling pathway in HCC |
Reviews
What customers say
All three reviews (average rating 4.7/5) are for Western Blot applications at a 1:1000 dilution. Users report clear, specific bands and excellent sensitivity. One reviewer used the antibody in cancer cell lines and praised its sensitivity. Another tested it in melanoma cell lines and noted it has been validated in published research. Overall, reviewers are highly satisfied with the antibody's performance in WB, highlighting its reliability for research and publication-quality results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Casey (Verified Customer) (11-30-2025) | Band was clear
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Haley (Verified Customer) (11-26-2025) | Great sensitivity
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Jelan (Verified Customer) (11-11-2025) | This is the antibody NCAPD2 which has been used in western blots by our lab and used in publications! Specifically for Co-Targeting FASN and mTOR Suppresses Uveal Melanoma Growth.
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