Tested Applications
| Positive WB detected in | COLO 320 cells, A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13396-1-AP targets B4GALNT1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4065 Product name: Recombinant human B4GALNT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 29-328 aa of BC029828 Sequence: DAPGLRLPLAPWAPPQSPRRPELPDLAPEPRYAHIPVRIKEQVVGLLAWNNCSCESSGGGLPLPFQKQVRAIDLTKAFDPAELRAASATREQEFQAFLSRSQSPADQLLIAPANSPLQYPLQGVEVQPLRSILVPGLSLQAASGQEVYQVNLTASLGTWDVAGEVTGVTLTGEGQADLTLVSPGLDQLNRQLQLVTYSSRSYQTNTADTGARPGWRDGQAGQTEKNQKGWSGQMAEGMGGIWAMARAVQPHNGCFNWTSRARGRKGAFVHLGLEQARGKPEPWVCLPFRPTVGGPRKRLV Predict reactive species |
| Full Name | beta-1,4-N-acetyl-galactosaminyl transferase 1 |
| Calculated Molecular Weight | 532 aa, 58 kDa |
| Observed Molecular Weight | 67.5 kDa |
| GenBank Accession Number | BC029828 |
| Gene Symbol | B4GALNT1 |
| Gene ID (NCBI) | 2583 |
| RRID | AB_2061077 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q00973 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for B4GALNT1 antibody 13396-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The B4GALNT1 Polyclonal antibody (Cat# 13396-1-AP) has 6 total citations. These appear in Nature Cell Biology, Cell Proliferation, iScience, Frontiers in Genetics, Journal of Gene Medicine, and Journal of Clinical Laboratory Analysis. The associated research fields span mitochondrial fission and metastatic chemotherapy resistance, Kupffer cell polarization in liver transplantation, NMDA receptor synaptic plasticity and cognition, and hepatocellular carcinoma involving epithelial-mesenchymal transition and PI3K-AKT-mTOR signaling.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Mitochondrial fission links ECM mechanotransduction to metabolic redox homeostasis and metastatic chemotherapy resistance. | ||
Cell Prolif SCARF1 promotes M2 polarization of Kupffer cells via calcium-dependent PI3K-AKT-STAT3 signalling to improve liver transplantation. | ||
Front Genet Identification of a Novel Glycosyltransferase Prognostic Signature in Hepatocellular Carcinoma Based on LASSO Algorithm. | ||
J Gene Med B4GALNT1 promotes carcinogenesis by regulating epithelial-mesenchymal transition in hepatocellular carcinoma based on pan-cancer analysis
| ||
J Clin Lab Anal B4GALNT1 Regulates Hepatocellular Carcinoma Cell Proliferation and Apoptosis via the PI3K-AKT-mTOR Pathway |
