Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13558-1-AP targets PNKP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4421 Product name: Recombinant human PNKP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 171-521 aa of BC033822 Sequence: DLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG Predict reactive species |
| Full Name | polynucleotide kinase 3'-phosphatase |
| Calculated Molecular Weight | 521 aa, 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC033822 |
| Gene Symbol | PNKP |
| Gene ID (NCBI) | 11284 |
| RRID | AB_2877958 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96T60 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PNKP antibody 13558-1-AP | Download protocol |
| WB protocol for PNKP antibody 13558-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PNKP polyclonal antibody (Cat# 13558-1-AP) has 1 citation to date. It was cited in Cell Signal (2025) in a study examining how macrophage-derived exosomal miR-146a-5p modulates the PNKP/DDOST/JAGN1 complex to regulate NETs formation in atherosclerosis. The antibody was used for Western blot in human samples. The associated research fields include atherosclerosis, exosome biology, and neutrophil extracellular trap (NET) regulation.
| Species | Application | Title |
|---|---|---|
Cell Signal Macrophage-derived exosome miR-146a-5p modulates PNKP/DDOST/JAGN1 complex to regulate NETs formation in atherosclerosis. |
Reviews
What customers say
The single review for this antibody reports excellent performance in Western Blot. The user detected PNKP in HEK293 cells using a 1:500 dilution and rated the product 5 out of 5 stars, describing it as working "extremely well." Overall, the feedback is highly positive, indicating reliable and strong detection for the intended application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Isabelle Cristine (Verified Customer) (01-14-2022) | This antibody works extremely well for detecting PNKP in HEK293 cells.
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